BioP Colour Bacteria - Revision history

Vanessa Lorenzo: /* Microbiology of pigment-producing bacteria */

2020-10-27T10:20:38Z

Microbiology of pigment-producing bacteria

Vanessa Lorenzo: /* Microbiology of pigment-producing bacteria */

2020-10-27T10:19:39Z

Microbiology of pigment-producing bacteria

Vanessa Lorenzo: /* Protocol: make your own experiment */

2016-12-19T12:47:05Z

Protocol: make your own experiment

Vanessa Lorenzo: /* Materials */

2016-12-19T12:45:18Z

Materials

Vanessa Lorenzo: /* Materials */

2016-12-19T12:44:59Z

Materials

Vanessa Lorenzo at 12:44, 19 December 2016

2016-12-19T12:44:29Z

Show changes

Vanessa Lorenzo at 12:32, 19 December 2016

2016-12-19T12:32:46Z

Vanessa Lorenzo at 12:32, 19 December 2016

2016-12-19T12:32:09Z

Vanessa Lorenzo at 12:25, 19 December 2016

2016-12-19T12:25:01Z

Vanessa Lorenzo at 12:19, 19 December 2016

2016-12-19T12:19:30Z

@@ Line 1: / Line 1: @@
 = Microbiology of pigment-producing bacteria=
-Conntribution to Open Science School’s Co-lab Biomaterial EPFL & Hackuarium, by Vanessa Lorenzo Toquero [http://www.hybridoa.org Hybridoa]
+Contribution to Open Science School’s Co-lab Biomaterial EPFL & Hackuarium, December 2016, by Vanessa Lorenzo Toquero [http://www.hybridoa.org Hybridoa][[File:BioInk4Colors.JPG | 400px | thumb| left l Bioink Colours ]]
 == Introduction ==

@@ Line 1: / Line 1: @@
 = Microbiology of pigment-producing bacteria=
-Open Science School’s Co-lab Biomaterial EPFL & Hackuarium
+Conntribution to Open Science School’s Co-lab Biomaterial EPFL & Hackuarium, by Vanessa Lorenzo Toquero [http://www.hybridoa.org Hybridoa]
 December 2016. EPFL & Hackuarium, Lausanne
 [[File:BioInk4Colors.JPG | 400px | thumb| left l Bioink Colours ]]

@@ Line 69: / Line 69: @@
 ::<li> Lb liquide + 1%Glycerol
-===Protocol: make your own experiment===
+==Protocol: make your own experiment==
 Now you are free to draw colorful painting on your petri dishes, or experiment and use the bacteria on different conditions to check their growth. Please note the results and anticipate the results beforehand as an exercise.
@@ Line 76: / Line 76: @@
 Pigments are normally produced by bacteria, plants, or even animals as a reaction to environmental stress or defense.
-====J. lividum====
+===J. lividum===
 For example, the pigment violacein from J. lividum inhibits the toxic effect and growth of a fungus known as Batrachochytrium. This fungus causes a disease known as Chytridiomycosis in amphibians The disease devastated amphibian populations around the world, in a global decline towards multiple extinctions, part of the Holocene extinction. Because of this, understanding the uses of this bacteria has been of major interest. A study conducted in 2009 explored the effects of Bd and the use of J. lividium in the lab for survival. They used three experimental treatments: frogs infected with Bd, frogs given the bacteria J. lividium and frogs with the given bacteria and then exposed to Bd. Nearly all of the frogs exposed to Bd experienced mortality while none of the other treatments had any deaths. This effectively introduced the use of J. lividium as a possible method for Bd prevention in the lab setting.
-Care: As you see, this bacteria can help you fight some diseases on amphibians (salamanders, frogs, etc). Lb agar plate, and a cozy 25 - 30 °C Temperature would make them happy. Purple babies will colonize your plate after 4 - 7 days. Wanna ink? The best is to transport them with a sterile spatula to a Lb (liquid) or Lb +1% glycerol (liquid) bottle, shake it constantly at around 270 rpm and a cozy 25 - 30 °C Temperature to make them happy.
+====Care====
-====Micrococcus Luteus====
+===Micrococcus Luteus===
 Yellow mates that can be found almost everywhere: in soil, dust, water, air, in our skin… they are strong and they can live (almost) forever. Does a time machine of 30.000 years tell you something? Also, they block UV radiation… let’s go to the beach!
-Care: Lb agar plate, and a warm up of 37°C Temperature for about 3 - 4 days would make them happy. Wanna ink? The best is to transport them with a sterile spatula to a Lb (liquid) shake it constantly at around 270 rpm at 37°C to have their shiniest bright yellow.
+====Care====
-====Micrococcus Roseus====
+===Micrococcus Roseus===
 Siblings of Luteus and living on mammalian skin, so, your skin as well! Uv protector and beautiful color pigment micro - factories. Are you thinking about a new tattoo?
-Care: Lb agar plate, and a warm up of 25 - 37°C (they are chill and relaxed about the temperature) for about 3 - 4 days would make them happy. Wanna ink? The best is to transport them with a sterile spatula to a Lb (liquid). Shake them constantly at around 270 rpm at 37°C to have their beautiful and elegant pink-salmon babies.
+====Care====
 == Activities ==

@@ Line 63: / Line 63: @@
 <br>
 ==Materials==
-Nutrient agar plates
+::<li> Nutrient agar plates
-Luria Broth plates
+::<li> Luria Broth plates
-Luria Broth plates with Ampicillin (100 mg/ml), IPTG (1 mM) and tryptophan (2 g/l)
+::<li> Luria Broth plates with Ampicillin (100 mg/ml), IPTG (1 mM) and tryptophan (2 g/l)
-Lb liquide
+::<li> Lb liquide
-Lb liquide + 1%Glycerol
+::<li> Lb liquide + 1%Glycerol
 ===Protocol: make your own experiment===
@@ Line 90: / Line 90: @@
 Care: Lb agar plate, and a warm up of 25 - 37°C (they are chill and relaxed about the temperature) for about 3 - 4 days would make them happy. Wanna ink? The best is to transport them with a sterile spatula to a Lb (liquid). Shake them constantly at around 270 rpm at 37°C to have their beautiful and elegant pink-salmon babies.
 == Activities ==

@@ Line 62: / Line 62: @@
 <br>
 <br>
-===Materials===
+==Materials==
 Nutrient agar plates
 Luria Broth plates

@@ Line 7: / Line 7: @@
 December 2016. EPFL & Hackuarium, Lausanne
-== Introduction ==
+= Introduction =
 ::<li> Growth conditions of microorganisms
 ::<li> Physical (temperature, light), chemical (pH, osmolarity), toxicity (antibiotic, heavy metal), or nutrient requirement (auxotrophy, bioavailability) conditions.
 ::<li> Pigments and physical nature of color.
 Bacterial growth is affected by (1) temperature, (2) nutrient availability, (3) water supply, (4) oxygen supply, and (5) acidity of the medium.
@@ Line 88: / Line 89: @@
 == Activities ==
-. Observe the different microorganisms
+::<li>  1. Observe the different microorganisms
-. Play with the bacteria printer
+::<li>  2. Play with the bacteria printer
-. Experiment different supports for their growth.
+::<li>  3. Experiment different supports for their growth.
-Experiment Kit
+==Experiment Kit==
-(10 Kits)
+(10 Kits * Experiment)
-Step one:
+===Kit 1===
 Mixed bacteria plate
 Spatula
@@ Line 102: / Line 103: @@
 Spot and isolate your colorful babies!
-Step two:
+===Experiment 1 description===
 Experiment Description
 Overall explanation of 1, 2, 3 steps. In groups of 3 people, choose 2 steps and 10 min experiment for 1st and 2nd step, 5min for 3rd step.
+<br>
 st step, clean your spatula,open the mixed color petri, take a sample of one of the colors at a time. Scatter them into one area of the petri dish.
 <br>
@@ Line 127: / Line 112: @@
 <br>
 <br>
 nd step, soak your brush in the bacterial ink and paint your paper.
 <br>
@@ Line 132: / Line 126: @@
 <br>
 <br>
 rd step, design your path and print with it.
 <br>
@@ Line 138: / Line 139: @@
 <br>
-Biosafety
+== General Material ==
 All the samples will stay at the lab and destroyed after 5 days (to see and document development), only the prototypes sprayed with ethanol or blitch will be kept.

@@ Line 125: / Line 125: @@
 st step, clean your spatula,open the mixed color petri, take a sample of one of the colors at a time. Scatter them into one area of the petri dish.
 <br>
-[[File:Scatter_Biopigments.png | Isolate ]]
+[[File:Scatter_Biopigments.png | 400px | Isolate ]]
 <br>
 <br>
 nd step, soak your brush in the bacterial ink and paint your paper.
 <br>
-[[File:Paint_Biopigments.png | Paint ]]
+[[File:Paint_Biopigments.png | 400px | Paint ]]
 <br>
 <br>
 rd step, design your path and print with it.
 <br>
-[[File:Print_Biopigments-01.png | Print ]]
+[[File:Print_Biopigments-01.png | 400px | Print ]]
 <br>
 <br>