Hekkcess: Created page with "File:GDR.png
== Introduction == ::
During a [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] process, often a [http://en.wikipedia.org/wiki/Plasmid pl..."

2015-01-17T13:12:19Z

Created page with "File:GDR.png == Introduction == ::<li> During a [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] process, often a [http://en.wikipedia.org/wiki/Plasmid pl..."

New page

[[File:GDR.png]]
 
== Introduction ==
::<li> During a [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] process, often a [http://en.wikipedia.org/wiki/Plasmid plasmid] is used and part of its sequence is amplified. After the PCR the plasmid is still present and has to be destroyed to avoid contamination further away in the process. This DNA is called [http://en.wikipedia.org/wiki/DNA_methylation methylated genomic DNA] (Me-DNA).
::<li> The dPNI enzyme is a [http://en.wikipedia.org/wiki/Restriction_enzyme#Type_II restriction enzyme] that will cut the Me-DNA
::<li> The buffer is a specific buffer used to give your enzyme the best operating conditions.
::<li> The enzyme has to operate at a specific temperature (often 37°C) and then be denatured (destroyed) by heating it (often 80°C) this is why we use a [http://www.thermoscientific.com/content/tfs/en/product/dry-block-heaters.html heat-block] or a PCR
 
== Protocols ==
 
== Safety ==
 
== Hackuarium Specifics ==

BioP GDR - Revision history

Hekkcess: Created page with "File:GDR.png == Introduction == :: During a [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] process, often a [http://en.wikipedia.org/wiki/Plasmid pl..."

Hekkcess: Created page with "File:GDR.png
== Introduction == ::
During a [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] process, often a [http://en.wikipedia.org/wiki/Plasmid pl..."