https://wiki.hackuarium.ch/index.php?action=history&feed=atom&title=BioP_transfection 2026-04-05T23:58:37Z Revision history for this page on the wiki MediaWiki 1.35.3 https://wiki.hackuarium.ch/index.php?title=BioP_transfection&diff=4506&oldid=prev 2015-01-17T13:24:04Z

<p>Created page with &quot;<a href="/w/File:Transfection.png" title="File:Transfection.png">800px</a> &lt;br&gt; == Introduction == ::&lt;li&gt;The transfection is the process of inserting a plasmid inside a bacteria. Several types of bacteria can be used a...&quot;</p> <p><b>New page</b></p><div>[[File:transfection.png|800px]]<br /> &lt;br&gt;<br /> == Introduction ==<br /> ::&lt;li&gt;The transfection is the process of inserting a plasmid inside a bacteria. Several types of bacteria can be used as host for the plasmid and will usually express the information contained in this plasmid afterwards.<br /> === Electro competent way ===<br /> ::&lt;li&gt; Here the bacteria is inserted with the plasmid inside a cuvette. The cuvette will be added to an electroporator and an electric current will be applied tearing holes in the bacteria forcing the plasmid inside.<br /> ::&lt;li&gt; The bacteria is transferred to an aliquot and the medium is added for it to recover in a heat-shaker.<br /> ::&lt;li&gt; The voltage is usually 1700V but can vary depending on the bacteria you use.<br /> === Chemo competent way ===<br /> ::&lt;li&gt; !!! HAS TO BE CORRECTED ON DRAWING AND TEXT HAS TO BE ADDED !!!<br /> <br /> &lt;br&gt;<br /> == Protocols ==<br /> &lt;br&gt;<br /> == Safety ==<br /> &lt;br&gt;<br /> == Hackuarium Specifics ==<br /> &lt;br&gt;</div>

Hekkcess