Bioluminescence investigations - Revision history

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2026-08-21T10:46:08Z

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2025-09-03T17:08:51Z

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2025-09-01T21:37:05Z

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2025-09-01T21:34:56Z

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2025-09-01T21:27:32Z

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Rachel at 21:23, 1 September 2025

2025-09-01T21:23:33Z

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2025-09-01T21:21:18Z

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 They ordered ''Vibrio fischeri'' from a French biotech company in [https://www.conatex.com/fr/catalog/svt/microbiologie/experiences_avec_des_micro_organismes/product-kit_bioluminescence/sku-1093487#.XMReN-szbwc a special kit].
-while a bit of light was visible in cultures, in comparison to controls, and determined by unbiased dark-adapted participants, it was not very bright, and no good pictures were obtained of it...<br>
+while a bit of light was visible in cultures, in comparison to controls, and determined by unbiased dark-adapted participants, it was not very bright, and no good pictures were obtained of it...
 Advice was requested from
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 Here are the PCR reaction and cycling conditions we use (with Q5 pol to allow proofreading and avoid errors)<br>
 master mix for 5 rxns (25ul total)<br>
-ul 5x Q5 buffer<br>
+ul 5x Q5 buffer <br>
 .375 ul H2O<br>
 .125ul 10mM dNTPs<br>

← Older revision		Revision as of 17:08, 3 September 2025
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	Clear effects of growth and luminescence were noted. Can see the link above for still more details...		Clear effects of growth and luminescence were noted. Can see the link above for still more details...
		+
		+	This [https://pmc.ncbi.nlm.nih.gov/articles/PMC6294908/ article talks about the genetics of mycelia bioluminescence]. Apparently, ''P. stipticus'' has just a Luciferase and an enzyme (h3h) to make the substrate luciferin.

@@ Line 93: / Line 93: @@
 For instance... in 2025 we got some bioluminescent mycelia from Ove Mattmann, ''Panellus stipticus'', and did some experiments with pH with a gymnase student, Lucie D, doing her TM on bioluminescence.  Here are some [https://docs.google.com/presentation/d/12R1h-IVZR3m5qv_aPtyRH_s-0a2QUC-Jy1U3kSrypcI/edit?usp=sharing first results].  Interestingly, not only did they require fresher malt extract for glowing on our plates, but their biolum comes out green on the same camera as the one I used for the dinoflagellates...[[File:Brightfield.png|thumb|to left,  P. stipticus: above, normal media, below acidic. to right, F. fomentarius, a non luminescent mycelia.]]
-[[File:Screenshotwithambientlight firstPositivepHeffect.png|alt=fades a week later|thumb|Screenshot of biolum from ''P. stipticus'' on plates with ambient light - our first Positive pH effect ]]The mycelia of the ''P. stipticus'' were sensitive to carmelized old maltose, and only brightened up in culture after we got a new batch, but pH tests were fun to initiate, with Citric Acid (1M) used for adjusting it.
+[[File:Screenshotwithambientlight firstPositivepHeffect.png|alt=fades a week later|thumb|Screenshot of biolum from ''P. stipticus'' on plates with ambient light - our first Positive pH effect ]]The mycelia of the ''P. stipticus'' were sensitive to carmelized old maltose, and only brightened up in culture after we got a new batch. For another TM the pH tests were fun to initiate, with Citric Acid (1M) used for adjusting it, with buffered solutions included for the liquid based tests...
 Clear effects of growth and luminescence were noted.  Can see the link above for still more details...

@@ Line 93: / Line 93: @@
 For instance... in 2025 we got some bioluminescent mycelia from Ove Mattmann, ''Panellus stipticus'', and did some experiments with pH with a gymnase student, Lucie D, doing her TM on bioluminescence.  Here are some [https://docs.google.com/presentation/d/12R1h-IVZR3m5qv_aPtyRH_s-0a2QUC-Jy1U3kSrypcI/edit?usp=sharing first results].  Interestingly, not only did they require fresher malt extract for glowing on our plates, but their biolum comes out green on the same camera as the one I used for the dinoflagellates...[[File:Brightfield.png|thumb|to left,  P. stipticus: above, normal media, below acidic. to right, F. fomentarius, a non luminescent mycelia.]]
-[[File:Screenshotwithambientlight firstPositivepHeffect.png|alt=fades a week later|thumb|Screenshot of biolum from ''P. stipticus'' on plates with ambient light - our first Positive pH effect ]]The mycelia of the ''P. stipticus'' were sensitive to carmelized old maltose, and only brightened up in culture after we got a new batch, but pH tests were fun to initiate, with Citric Acid (1M) used for adjusting.
+[[File:Screenshotwithambientlight firstPositivepHeffect.png|alt=fades a week later|thumb|Screenshot of biolum from ''P. stipticus'' on plates with ambient light - our first Positive pH effect ]]The mycelia of the ''P. stipticus'' were sensitive to carmelized old maltose, and only brightened up in culture after we got a new batch, but pH tests were fun to initiate, with Citric Acid (1M) used for adjusting it.
 Clear effects of growth and luminescence were noted.  Can see the link above for still more details...

@@ Line 92: / Line 92: @@
 more results to come.
-For instance... in 2025 we got some bioluminescent mycelia from Ove Mattmann, ''Panellus stipticus'', and did some experiments with pH with a gymnase student, Lucie D, doing her TM on bioluminescence.  Here are some [https://docs.google.com/presentation/d/12R1h-IVZR3m5qv_aPtyRH_s-0a2QUC-Jy1U3kSrypcI/edit?usp=sharing first results].  Interestingly, not only did they require fresher malt extract for glowing on our plates, but their biolum comes out green on the same camera as the one I used for the dinoflagellates...[[File:Brightfield.png|thumb|]]
+For instance... in 2025 we got some bioluminescent mycelia from Ove Mattmann, ''Panellus stipticus'', and did some experiments with pH with a gymnase student, Lucie D, doing her TM on bioluminescence.  Here are some [https://docs.google.com/presentation/d/12R1h-IVZR3m5qv_aPtyRH_s-0a2QUC-Jy1U3kSrypcI/edit?usp=sharing first results].  Interestingly, not only did they require fresher malt extract for glowing on our plates, but their biolum comes out green on the same camera as the one I used for the dinoflagellates...[[File:Brightfield.png|thumb|to left,  P. stipticus: above, normal media, below acidic. to right, F. fomentarius, a non luminescent mycelia.]]
-[[File:Screenshotwithambientlight firstPositivepHeffect.png|alt=fades a week later|thumb|Screenshot of biolum from ''P. stipticus'' on plates with ambient light - our first Positive pH effect ]]
+[[File:Screenshotwithambientlight firstPositivepHeffect.png|alt=fades a week later|thumb|Screenshot of biolum from ''P. stipticus'' on plates with ambient light - our first Positive pH effect ]]The mycelia of the ''P. stipticus'' were sensitive to carmelized old maltose, and only brightened up in culture after we got a new batch, but pH tests were fun to initiate, with Citric Acid (1M) used for adjusting.

← Older revision		Revision as of 21:24, 1 September 2025
Line 93:		Line 93:

	For instance... in 2025 we got some bioluminescent mycelia from Ove Mattmann, ''Panellus stipticus'', and did some experiments with pH with a gymnase student, Lucie D, doing her TM on bioluminescence. Here are some [https://docs.google.com/presentation/d/12R1h-IVZR3m5qv_aPtyRH_s-0a2QUC-Jy1U3kSrypcI/edit?usp=sharing first results]. Interestingly, not only did they require fresher malt extract for glowing on our plates, but their biolum comes out green on the same camera as the one I used for the dinoflagellates...		For instance... in 2025 we got some bioluminescent mycelia from Ove Mattmann, ''Panellus stipticus'', and did some experiments with pH with a gymnase student, Lucie D, doing her TM on bioluminescence. Here are some [https://docs.google.com/presentation/d/12R1h-IVZR3m5qv_aPtyRH_s-0a2QUC-Jy1U3kSrypcI/edit?usp=sharing first results]. Interestingly, not only did they require fresher malt extract for glowing on our plates, but their biolum comes out green on the same camera as the one I used for the dinoflagellates...
−	[File:Screenshotwithambientlight firstPositivepHeffect.png\|alt=fades a week later\|thumb\|Screenshot of biolum from ''P. stipticus'' on plates with ambient light - our first Positive pH effect ]]	+	[[File:Screenshotwithambientlight firstPositivepHeffect.png\|alt=fades a week later\|thumb\|Screenshot of biolum from ''P. stipticus'' on plates with ambient light - our first Positive pH effect ]]

← Older revision		Revision as of 21:23, 1 September 2025
Line 93:		Line 93:

	For instance... in 2025 we got some bioluminescent mycelia from Ove Mattmann, ''Panellus stipticus'', and did some experiments with pH with a gymnase student, Lucie D, doing her TM on bioluminescence. Here are some [https://docs.google.com/presentation/d/12R1h-IVZR3m5qv_aPtyRH_s-0a2QUC-Jy1U3kSrypcI/edit?usp=sharing first results]. Interestingly, not only did they require fresher malt extract for glowing on our plates, but their biolum comes out green on the same camera as the one I used for the dinoflagellates...		For instance... in 2025 we got some bioluminescent mycelia from Ove Mattmann, ''Panellus stipticus'', and did some experiments with pH with a gymnase student, Lucie D, doing her TM on bioluminescence. Here are some [https://docs.google.com/presentation/d/12R1h-IVZR3m5qv_aPtyRH_s-0a2QUC-Jy1U3kSrypcI/edit?usp=sharing first results]. Interestingly, not only did they require fresher malt extract for glowing on our plates, but their biolum comes out green on the same camera as the one I used for the dinoflagellates...
−	~~[[File:Biolum_Pstipticus.png\|thumb\|]]~~
	[File:Screenshotwithambientlight firstPositivepHeffect.png\|alt=fades a week later\|thumb\|Screenshot of biolum from ''P. stipticus'' on plates with ambient light - our first Positive pH effect ]]		[File:Screenshotwithambientlight firstPositivepHeffect.png\|alt=fades a week later\|thumb\|Screenshot of biolum from ''P. stipticus'' on plates with ambient light - our first Positive pH effect ]]

← Older revision		Revision as of 21:21, 1 September 2025
Line 93:		Line 93:

	For instance... in 2025 we got some bioluminescent mycelia from Ove Mattmann, ''Panellus stipticus'', and did some experiments with pH with a gymnase student, Lucie D, doing her TM on bioluminescence. Here are some [https://docs.google.com/presentation/d/12R1h-IVZR3m5qv_aPtyRH_s-0a2QUC-Jy1U3kSrypcI/edit?usp=sharing first results]. Interestingly, not only did they require fresher malt extract for glowing on our plates, but their biolum comes out green on the same camera as the one I used for the dinoflagellates...		For instance... in 2025 we got some bioluminescent mycelia from Ove Mattmann, ''Panellus stipticus'', and did some experiments with pH with a gymnase student, Lucie D, doing her TM on bioluminescence. Here are some [https://docs.google.com/presentation/d/12R1h-IVZR3m5qv_aPtyRH_s-0a2QUC-Jy1U3kSrypcI/edit?usp=sharing first results]. Interestingly, not only did they require fresher malt extract for glowing on our plates, but their biolum comes out green on the same camera as the one I used for the dinoflagellates...
		+	[[File:Biolum_Pstipticus.png\|thumb\|]]
		+	[File:Screenshotwithambientlight firstPositivepHeffect.png\|alt=fades a week later\|thumb\|Screenshot of biolum from ''P. stipticus'' on plates with ambient light - our first Positive pH effect ]]