DIY Fluorescence Microscope - Revision history

Vithoo: /* Material Used */

2018-09-04T13:42:22Z

Material Used

2018-09-04T13:41:37Z

Material Used

2018-09-04T13:41:15Z

Material Used

2018-09-04T13:18:06Z

2018-09-04T08:33:01Z

2018-09-04T08:31:48Z

No more used

2018-09-04T08:31:28Z

2018-09-04T08:15:24Z

Results

2018-09-04T08:13:15Z

Results

2018-09-04T08:12:37Z

Results

@@ Line 7: / Line 7: @@
 ==Material Used==
-We get inspiration from the DIY Gel Transiluminator build by the GaudiLabs. ([[Diy-transilluminator]]) We observe that this equipment allows us to observe reactions done with the SYBRsafe (?) and thus decided to mimic it. In short, blue LEDs are used for the excitation part. Then a yellow lens is added to remove specific wavelength and keep only that one of interest.
+We get inspiration from the [[Diy-transilluminator]]. We observe that this equipment allows us to observe reactions done with the SYBRsafe (?) and thus decided to mimic it. In short, blue LEDs are used for the excitation part. Then a yellow lens is added to remove specific wavelength and keep only that one of interest.
 ==Results ==

@@ Line 7: / Line 7: @@
 ==Material Used==
-We get inspiration from the DIY Gel Transiluminator build by the GaudiLabs. (http://wiki.hackuarium.ch/w/Diy-transilluminator) We observe that this equipment allows us to observe reactions done with the SYBRsafe (?) and thus decided to mimic it. In short, blue LEDs are used for the excitation part. Then a yellow lens is added to remove specific wavelength and keep only that one of interest.
+We get inspiration from the DIY Gel Transiluminator build by the GaudiLabs. ([[Diy-transilluminator]]) We observe that this equipment allows us to observe reactions done with the SYBRsafe (?) and thus decided to mimic it. In short, blue LEDs are used for the excitation part. Then a yellow lens is added to remove specific wavelength and keep only that one of interest.
 ==Results ==

@@ Line 7: / Line 7: @@
 ==Material Used==
-We get inspiration from the DIY Gel Transiluminator build by the GaudiLabs. We observe that this equipment allows us to observe reactions done with the SYBRsafe (?) and thus decided to mimic it. In short, blue LEDs are used for the excitation part. Then a yellow lens is added to remove specific wavelength and keep only that one of interest.
+We get inspiration from the DIY Gel Transiluminator build by the GaudiLabs. (http://wiki.hackuarium.ch/w/Diy-transilluminator) We observe that this equipment allows us to observe reactions done with the SYBRsafe (?) and thus decided to mimic it. In short, blue LEDs are used for the excitation part. Then a yellow lens is added to remove specific wavelength and keep only that one of interest.
 ==Results ==

@@ Line 1: / Line 1: @@
-Before the observation of fluorescent process was not possible in the hacker space. It was thus decided that transforming one of the microscope into a fluorescent microscope would be cool.
+In order to observe the result from the [[Comet Cell Assay 2018]], SYBRsafe is added to color the tails. Before the observation of fluorescent process was not possible in the hacker space. It was thus decided to transform one of the microscope into a fluorescent microscope.
-==Background and Procedure==
+==Biological Background==
 ==Results ==

@@ Line 25: / Line 25: @@
 We observe that the resulting "phase" image in yellowish, this is due to the yellow lens that have been added. An idea would be to remove this lens to take the "phase" picture and add it only for the "fluo" one.
-==Available Code==
+===Available Code===
 ==No more used==
 This microscope works well to observe fluorescent nucleus but its resolution is not enough to observe comets. Thus it could not be used to assess the [[Comet Cell Assay 2018]].

← Older revision		Revision as of 08:31, 4 September 2018
Line 29:		Line 29:
	==No more used==		==No more used==

−	This microscope works well to observe fluorescent nucleus but its resolution is not enough to observe comets. Thus it could not be used to assess the [[Comet Cell Assay 2018]].	+	This microscope works well to observe fluorescent nucleus but its resolution is not enough to observe comets. Thus it could not be used to assess the [[Comet Cell Assay 2018]].

← Older revision		Revision as of 08:31, 4 September 2018
Line 26:		Line 26:

	==Available Code==		==Available Code==
		+
		+	==No more used==
		+
		+	This microscope works well to observe fluorescent nucleus but its resolution is not enough to observe comets. Thus it could not be used to assess the [[Comet Cell Assay 2018]].

@@ Line 4: / Line 4: @@
 ==Results ==
 Fluorescence is visible through the Raspberry Pi Camera. An idea then is to implement a code to overlay the picture of the cell and the fluorescent picture. The goal of this overlay would be to confirm that the fluorescence comes from the nucleus.
@@ Line 18: / Line 20: @@
 File:Overlay2.png|Overlay
 </gallery>
 ==Available Code==

@@ Line 9: / Line 9: @@
 <gallery perrow="5" mode="packed" widths="px" heights="px">
 File:16-29-06.jpg|Phase
-File:10-14-05.jpg|Fluo
+File:16-28-53.jpg|Fluo
 File:Overlay1.png|Overlay
 </gallery>