HTGAA - Revision history

Rachel: /* wk2: Bio Design / Plasmid digest */

2017-11-21T10:15:25Z

wk2: Bio Design / Plasmid digest

2017-11-21T10:14:43Z

wk2: Bio Design / Plasmid digest

2017-11-21T10:14:15Z

wk2: Bio Design / Plasmid digest

2017-10-27T12:29:24Z

Week 3 next generation synthesis

2017-10-27T12:29:01Z

Week 3 next generation synthesis

2017-10-16T14:48:10Z

wk2: Bio Design / Plasmid digest

2017-10-16T14:44:35Z

wk2: Bio Design / Plasmid digest

2017-10-16T14:44:09Z

2017-10-13T19:01:09Z

2017-10-13T15:05:23Z

@@ Line 64: / Line 64: @@
 [[File:IMG 1350.PNG |200px|]]
-plasmid ypp 3-3 (Yann Pierson's) was used for the test digests, and the further lanes on this 1% Agarose mini-gel were:
+The plasmid ypp 3-3 (Yann Pierson's) was used for these test digests, and the further lanes on this 1% Agarose mini-gel were:
 uncut (no enzyme, but incubated) <br>
 KpnI digest (should linearize for 6.48Kb band, but got partial digest) <br>
@@ Line 70: / Line 70: @@
 SacI digest (should linearize also) <br>
 KpnI+SacI (for double digest, expected 1.3 & 5.2Kb) <br>
 === wk3: next generation synthesis ===

@@ Line 62: / Line 62: @@
 Size markers, are in the first lane...
-[[File:IMG 1350.PNG |400px|]]
+[[File:IMG 1350.PNG |200px|]]
 plasmid ypp 3-3 (Yann Pierson's) was used for the test digests, and the further lanes on this 1% Agarose mini-gel were:

@@ Line 58: / Line 58: @@
 [[File:Gel_Hack_HTGAA.jpg |400px|]]
-The Gel contained:
+The Gel result was pretty good!
 === wk3: next generation synthesis ===

@@ Line 60: / Line 60: @@
 The Gel contained:
-=== Week 3 next generation synthesis ===
+=== wk3: next generation synthesis ===
 Theory task: design a gene with a detailed workflow of how to build it.

@@ Line 60: / Line 60: @@
 The Gel contained:
-== Week 3 next generation synthesis ==
+=== Week 3 next generation synthesis ===
 Theory task: design a gene with a detailed workflow of how to build it.
@@ Line 67: / Line 67: @@
 Ideally this task should be started ASAP as soon as the oligos arrive for the golden gate assembly
 == Headline text ==

@@ Line 55: / Line 55: @@
 The experiment was carried out on wednesday, 11th of of October 2017 with the help of Rachel.
-[[File:Gel_Hack_HTGAA.jpg]]
+[[File:Gel_Hack_HTGAA.jpg |400px|]
 The gel prepared was 20 mL of 1% agarose -1x TAE buffered gel. It was prepared by dissolving 0.2g of agarose inside 20 mL of a 1x TAE buffer solution. Upon boiling of the gel, SYBR safe dye was added and the liquid cast into the makeshift box.

← Older revision		Revision as of 19:01, 13 October 2017
Line 59:		Line 59:
	The gel prepared was 20 mL of 1% agarose -1x TAE buffered gel. It was prepared by dissolving 0.2g of agarose inside 20 mL of a 1x TAE buffer solution. Upon boiling of the gel, SYBR safe dye was added and the liquid cast into the makeshift box.		The gel prepared was 20 mL of 1% agarose -1x TAE buffered gel. It was prepared by dissolving 0.2g of agarose inside 20 mL of a 1x TAE buffer solution. Upon boiling of the gel, SYBR safe dye was added and the liquid cast into the makeshift box.
	The Gel contained:		The Gel contained:
		+
		+
		+	== Week 3 next generation synthesis ==
		+
		+	Theory task: design a gene with a detailed workflow of how to build it.
		+
		+	Lab task: Recode a GFP plasmid to another variant of fluorescent protein using a golden gate assembly.
		+
		+	Ideally this task should be started ASAP as soon as the oligos arrive for the golden gate assembly
		+
		+
		+
		+	== Headline text ==

← Older revision		Revision as of 15:05, 13 October 2017
Line 52:		Line 52:

	https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w/edit?usp=sharing		https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w/edit?usp=sharing
		+
		+	The experiment was carried out on wednesday, 11th of of October 2017 with the help of Rachel.
		+
		+	[[File:Gel_Hack_HTGAA.jpg]]
		+
		+	The gel prepared was 20 mL of 1% agarose -1x TAE buffered gel. It was prepared by dissolving 0.2g of agarose inside 20 mL of a 1x TAE buffer solution. Upon boiling of the gel, SYBR safe dye was added and the liquid cast into the makeshift box.
		+	The Gel contained: