Hypo proj - Revision history

Hekkcess: /* Protein */

2016-08-29T21:04:16Z

Protein

2016-08-29T21:04:02Z

Plasmid

2016-08-29T21:01:54Z

Design

2016-08-28T16:14:19Z

Design

2016-08-28T16:12:55Z

Summary

2016-08-28T16:12:03Z

Design

2016-08-28T16:08:49Z

Primers

2016-08-28T16:08:14Z

Primers

2016-08-28T16:06:59Z

Design

2016-08-28T16:01:42Z

Inserts

@@ Line 165: / Line 165: @@
 [[File:Temp_proj.png|400px|thumb|center|final plasmid design]]
-=Protein=
+==Protein==
 *Construct name:joh_j41_c (c01A4)
 *Sequence (coding frame only):

@@ Line 7: / Line 7: @@
 ==Date==
 .02.2111
-=Plasmid=
+=Design=
-== Design ==
+==Plasmid==
 *Name: joh_j41_p
 *Len: 6940bp
@@ Line 164: / Line 164: @@
 === Summary ===
 [[File:Temp_proj.png|400px|thumb|center|final plasmid design]]
 =Protein=
 *Construct name:joh_j41_c (c01A4)

← Older revision		Revision as of 21:01, 29 August 2016
Line 164:		Line 164:
	=== Summary ===		=== Summary ===
	[[File:Temp_proj.png\|400px\|thumb\|center\|final plasmid design]]		[[File:Temp_proj.png\|400px\|thumb\|center\|final plasmid design]]
		+	=Protein=
		+	*Construct name:joh_j41_c (c01A4)
		+	*Sequence (coding frame only):
		+	<div style="height:10em; overflow:auto; border: 2px solid #088">
		+	MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATNGKLTLKFICTT
		+	GKLPVPWPTLVTTFTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISF
		+	KDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNV
		+	YITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHY
		+	LSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKRPAANDENYAAS
		+	VMFKGIVEGIGIIEKIDIYTDLDKYAIRFPENMLNGIKKESSIMFNGCFLT
		+	VTSVNSNIVWFDIFEKEARKLDTFREYKVGDRVNLGTFPKFGAASGGHIL
		+	SARISCVASIIEIIENEDYQQMWIQIPENFTEFLIDKDYIAVDGISLTID
		+	TIKNNQFFISLPLKIAQNTNMKWRKKGDKVNVELSNKINANQCWGSGGAT
		+	HHHHHHH
		+	</div>
		+	*Len: 458 AA
		+	*Molecular Weight: 51734.61 Da
		+	*Extinction coefficient: 54110

@@ Line 9: / Line 9: @@
 =Plasmid=
 == Design ==
-Name: joh_j41_p
+*Name: joh_j41_p
-Len: 6940bp
+*Len: 6940bp
-Expression frame: 1854bp
+*Expression frame: 1854bp
 === Vector ===
 *Construction based on pET-51b(+)<br>

@@ Line 163: / Line 163: @@
 |}
 === Summary ===
-[[File:Temp_proj.png|200px|thumb|center|alt text]]
+[[File:Temp_proj.png|400px|thumb|center|final plasmid design]]

@@ Line 142: / Line 142: @@
 </div>
 ::Len : 753bp
-==Primers==
+===Primers===
 {| class="wikitable"
 |-

@@ Line 143: / Line 143: @@
 ::Len : 753bp
 ==Primers==
-{| class=wikitable border=1 width=330px cellpadding=7px
+{| class="wikitable"
-! Name | Sequence | Tm (°C)>
+|-
 |-
 | joh_j41_a1(a019E) || ACCGGCATATTTTATCGGTACTGGCTTTCCATTCGAC || 67

← Older revision		Revision as of 16:06, 28 August 2016
Line 142:		Line 142:
	</div>		</div>
	::Len : 753bp		::Len : 753bp
		+	==Primers==
		+	{\| class=wikitable border=1 width=330px cellpadding=7px
		+	! Name \| Sequence \| Tm (°C)>
		+	\|-
		+	\| joh_j41_a1(a019E) \|\| ACCGGCATATTTTATCGGTACTGGCTTTCCATTCGAC \|\| 67
		+	\|-
		+	\| joh_j41_a2(a019F)\|\| AGTACCGATAAAATATGCCGGTGTCAGTTCGGTAC \|\| 67
		+	\|-
		+	\| joh_j41_a3(a01A0) \|\| TGTTGAAAATGAAGGATCCGAAATCGGTACTGGCTTTC \|\| 68
		+	\|-
		+	\| joh_j41_a4(a01A1) \|\| TTCGGATCCTTCATTTTCAACAACATCAACGCTTGCAAC \|\| 67
		+	\|-
		+	\| joh_j41_a5(a01A2) \|\| TGGTAGTGAAGCAAGCGCCAGCATTATTG \|\| 66
		+	\|-
		+	\| joh_j41_a6(a01A3) \|\| AATGCTGGCGCTTGCTTCACTACCACCGCTGCCG \|\| 66
		+	\|}

@@ Line 123: / Line 123: @@
 </div>
 ::Len : 969bp
-*<span style="color:yellow">Insert 2:</span>
+*<span style="color:orange">Insert 2:</span>
 ::Name: joh_j41_dI2(d019D)
 ::Sequence: