Instru PCR - Revision history

Luchenry: /* Introduction */

2015-07-20T07:20:28Z

Introduction

Shalf_old at 16:42, 19 July 2015

2015-07-19T16:42:08Z

Shalf_old at 16:40, 19 July 2015

2015-07-19T16:40:02Z

Randogp: /* How to operate */

2015-07-19T16:10:39Z

How to operate

Randogp: /* How to operate */

2015-07-19T16:07:27Z

How to operate

Randogp: /* How to operate */

2015-07-19T15:07:58Z

How to operate

Hekkcess at 08:41, 17 June 2015

2015-06-17T08:41:26Z

Hekkcess: Created page with "== Introduction == The PCR or thermocycler instrument is a vital part in cloning and DNA amplification. Thanks to its versatile thermoblock the PCR can also be used for variou..."

2015-06-17T08:29:38Z

Created page with "== Introduction == The PCR or thermocycler instrument is a vital part in cloning and DNA amplification. Thanks to its versatile thermoblock the PCR can also be used for variou..."

New page

== Introduction ==
The PCR or thermocycler instrument is a vital part in cloning and DNA amplification.
Thanks to its versatile thermoblock the PCR can also be used for various, precise, heating and cooling actions.

==Our Model ==
Our model is a Tetrad PTC-225 Thermal Cycler. <br>
This model has the great advantage of operating independently 4 blocks simultaneously. <br>
The blocks can be changed for a 96 or 384 well format and are easy to swap.
[[File:PCR_I_1.jpg|300px|thumb|left|Thermocycler overview]]
[[File:PCR_I_2.jpg|300px|thumb|left|Power Unit]]
==Presentation==
::A) 384 well block
::B) 96 well block
::C) Block Status Display (Will tell you which unit is in operation, hot or dysfunctional)
::D) Display (you can read and program the PCR here)
::E) Keyboard (Every input can be made from here)
::F) "power" button ... This one does not work but we figured out something ;-)
::G) Power switch (the real power button)
::H) Power unit status LED

@@ Line 1: / Line 1: @@
 == Introduction ==
-The PCR or thermocycler instrument is a vital part in cloning and DNA amplification. <br>
+The PCR - aka thermocycler - instrument is a vital part of the molecular biology toolkit. Routinely used in cloning experiments for DNA amplification. <br>
 Thanks to its versatile thermoblock the PCR can also be used for various, precise, heating and cooling actions.

← Older revision		Revision as of 16:42, 19 July 2015
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	[[Category:Instruments]]		[[Category:Instruments]]
		+	[[Category:Open Food DNA]]

← Older revision		Revision as of 16:40, 19 July 2015
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	:::4.1) RUN a protocol: Both the underscore "_" and CAPITAL letters indicate the active command. Move the "_" on RUN using Select buttons. Push Proceed button. A standard PCR is on Yann_STD protocol. Push Proceed button. The next screen just confirms that you will run the Yann_STD protocol on block 1. Push Proceed button. Use heated lid? Say _YES. The heated lid prevents evaporation of the liquid sample.		:::4.1) RUN a protocol: Both the underscore "_" and CAPITAL letters indicate the active command. Move the "_" on RUN using Select buttons. Push Proceed button. A standard PCR is on Yann_STD protocol. Push Proceed button. The next screen just confirms that you will run the Yann_STD protocol on block 1. Push Proceed button. Use heated lid? Say _YES. The heated lid prevents evaporation of the liquid sample.
	[[File:Protocol_RUN.png]]		[[File:Protocol_RUN.png]]
		+
		+	[[Category:Instruments]]

← Older revision		Revision as of 16:10, 19 July 2015
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	::4) You can now either RUN, EDIT or make a NEW protocol.		::4) You can now either RUN, EDIT or make a NEW protocol.
	:::4.1) RUN a protocol: Both the underscore "_" and CAPITAL letters indicate the active command. Move the "_" on RUN using Select buttons. Push Proceed button. A standard PCR is on Yann_STD protocol. Push Proceed button. The next screen just confirms that you will run the Yann_STD protocol on block 1. Push Proceed button. Use heated lid? Say _YES. The heated lid prevents evaporation of the liquid sample.		:::4.1) RUN a protocol: Both the underscore "_" and CAPITAL letters indicate the active command. Move the "_" on RUN using Select buttons. Push Proceed button. A standard PCR is on Yann_STD protocol. Push Proceed button. The next screen just confirms that you will run the Yann_STD protocol on block 1. Push Proceed button. Use heated lid? Say _YES. The heated lid prevents evaporation of the liquid sample.
		+	[[File:Protocol_RUN.png]]

@@ Line 25: / Line 25: @@
 ::3) Place your samples in the unit of your choice. Make sure the lid fit tightly on the caps (see [http://youtu.be/PBHi0MVg9vA Demo video])
 ::4) You can now either RUN, EDIT or make a NEW protocol.
-:::4.1) RUN a protocol:
+:::4.1) RUN a protocol: Both the underscore "_" and CAPITAL letters indicate the active command. Move the "_" on RUN using Select buttons. Push Proceed button. A standard PCR is on Yann_STD protocol. Push Proceed button. The next screen just confirms that you will run the Yann_STD protocol on block 1. Push Proceed button. Use heated lid? Say _YES. The heated lid prevents evaporation of the liquid sample.

@@ Line 1: / Line 1: @@
 == Introduction ==
-The PCR or thermocycler instrument is a vital part in cloning and DNA amplification.
+The PCR or thermocycler instrument is a vital part in cloning and DNA amplification. <br>
 Thanks to its versatile thermoblock the PCR can also be used for various, precise, heating and cooling actions.
 ==Our Model ==
 Our model is a Tetrad PTC-225 Thermal Cycler. <br>
-This model has the great advantage of operating independently 4 blocks simultaneously. <br>
+This model has the great advantage of operating independently 4 blocks ( "Alpha unit" ) simultaneously. <br>
 The blocks can be changed for a 96 or 384 well format and are easy to swap.
 [[File:PCR_I_1.jpg|300px|thumb|left|Thermocycler overview]]
 [[File:PCR_I_2.jpg|300px|thumb|left|Power Unit]]
 ==Presentation==
 ::A) 384 well block
 ::B) 96 well block
 ::C) Block Status Display (Will tell you which unit is in operation, hot or dysfunctional)
@@ Line 18: / Line 18: @@
 ::G) Power switch (the real power button)
 ::H) Power unit status LED