Mycelium Leather - Revision history

Louli at 16:00, 14 June 2023

2023-06-14T16:00:56Z

Louli at 20:45, 14 May 2023

2023-05-14T20:45:12Z

Louli: /* Results */

2023-05-14T20:41:14Z

Results

Louli: /* Experimenting with substrates in petri dishes */ Day 12. Two zone hypotheses

2023-05-14T20:20:39Z

Experimenting with substrates in petri dishes: Day 12. Two zone hypotheses

Louli at 22:04, 13 May 2023

2023-05-13T22:04:31Z

Louli at 17:59, 13 May 2023

2023-05-13T17:59:17Z

Louli at 17:56, 13 May 2023

2023-05-13T17:56:00Z

Louli at 15:54, 10 May 2023

2023-05-10T15:54:49Z

Louli at 21:06, 6 May 2023

2023-05-06T21:06:19Z

Louli at 15:13, 6 May 2023

2023-05-06T15:13:29Z

@@ Line 36: / Line 36: @@
 {| class="wikitable"
 |+
-|dry beech wood shavings
+|dry beech (FR: hêtre) wood shavings
 |1 kg
 |-

@@ Line 207: / Line 207: @@
 # It first grew fast due to the grain energy but then slowed down, and slower growth could allow more thickness.  Test as before, also comparing the growth speed. Another test can be calculating the growth speed from pictures.
 # It oscillates between thick and thin, as [[User:Rachel|Rachel]] observed on another mycelium in agar filled petri dishes.  Test by waiting to see if another thin layer forms after the thick one.
-# Old mycelium has no purpose anymore and gives some of its mass to growing mycelium.
+# Old mycelium has no purpose anymore and gives some of its mass to growing mycelium. Test by looking if the zone interface moves forward.
 ===Heated inoculation chamber===

@@ Line 206: / Line 206: @@
 # It first had to get used to wood again, since the grain spawn was popcorn, bird seeds and liquid culture based on malt exctract. This would explain why it doesn't happen on agar: malt extract is more similar to grains than to wood.  Test by inoculating one wood dish with grain spawn and one with a piece of the thick zone. Compare the thickness of the growing mycelium.
 # It first grew fast due to the grain energy but then slowed down, and slower growth could allow more thickness.  Test as before, also comparing the growth speed. Another test can be calculating the growth speed from pictures.
-# Maybe it oscillates between thick and thin, as [[User:Rachel|Rachel]] observed on another mycelium in agar filled petri dishes.  Test by waiting to see if another thin layer forms after the thick one.
+# It oscillates between thick and thin, as [[User:Rachel|Rachel]] observed on another mycelium in agar filled petri dishes.  Test by waiting to see if another thin layer forms after the thick one.
 ===Heated inoculation chamber===

@@ Line 79: / Line 79: @@
 !Gypsum
 !''8 days''
 |-
 |1
@@ Line 87: / Line 88: @@
+|
 |''very bad''
 |-
 |2
@@ Line 95: / Line 97: @@
+|
 |good
 |-
 |3
@@ Line 103: / Line 107: @@
+|
 |very bad
 |-
 |4
@@ Line 111: / Line 116: @@
+|
 |bad
 |-
 |5
@@ Line 119: / Line 125: @@
 |0.025g
 |bad
 |-
 |6
@@ Line 127: / Line 134: @@
+|
 |very bad
 |-
 |7
@@ Line 135: / Line 143: @@
+|
 |very bad
 |-
 |8
@@ Line 143: / Line 152: @@
 |0.025g
 |bad
 |-
 |9
+|
+|
@@ Line 159: / Line 170: @@
+|
 |good
 |-
 |11
@@ Line 167: / Line 179: @@
+|
 |very good
 |-
 |12
@@ Line 175: / Line 188: @@
+|
 |very good
 |-
 | 13
@@ Line 183: / Line 197: @@
 |0.025g
 |very good
 |}
 To accurately measure those values I dissolved the nutrients in water solutions and used a pipetter. However gypsum is not soluble so the quantities may be inaccurate, and Maizena clumped together, so I put clumps of it into the petri dishes.
 ===Heated inoculation chamber===

@@ Line 32: / Line 32: @@
 |Spawn
 |288 g
 |}

@@ Line 172: / Line 172: @@
 ===A very cheap CO<sub>2</sub> meter===
-Since an electronic CO<sub>2</sub> sensor costs 60 CHF I make an analog one. Henry's law states that the amount of a dissolved gas in water is proportional to the partial pressure in the air above the water. Furthermore, dissolved CO<sub>2</sub> lowers the ph of water. This means that by filling a small bowl with distilled water and measuring its pH we can guess the CO<sub>2</sub> concentration of the air. Since the math are complicated, we just assume a linear (or exponential) relation and calibrate with the known atmospheric concentration and gas mixtures with CO<sub>2</sub> made from baking soda and vinegar.
+Since an electronic CO<sub>2</sub> sensor costs 60 CHF I make an analog one. Henry's law states that the amount of a dissolved gas in water is proportional to the partial pressure in the air above the water. Furthermore, dissolved CO<sub>2</sub> lowers the ph of water. This means that by filling a small bowl with distilled water and adding a few drops of pH indicator liquid we use its color to guess the CO<sub>2</sub> concentration of the air. Since the math are complicated, we just assume a linear relation and calibrate with CO<sub>2</sub> made from baking soda and vinegar and an expensive sensor.
 '''''[CO<sub>2</sub>] = a(T) + b(T)*pH'''''
 <nowiki><references \></nowiki>

@@ Line 51: / Line 51: @@
 ===Experimenting with substrates in petri dishes===
-I filled petri dishes with Agar, malt extract, maizena, yeast extract and gypsum according to the following table.
+On the 2.5.2023 I filled petri dishes with Agar, malt extract, maizena, yeast extract and gypsum according to the following table.
 {| class="wikitable mw-collapsible mw-collapsed"
 |+Nutrient tests on petri dishes
@@ Line 60: / Line 60: @@
 !Yeast
 !Gypsum
 |-
 |1
@@ Line 67: / Line 68: @@
+|
+|
 |-
 |2
@@ Line 74: / Line 76: @@
+|
+|
 |-
 |3
@@ Line 81: / Line 84: @@
 |0.05 g
+|
 |-
 |4
@@ Line 88: / Line 92: @@
 |0.05 g
+|
 |-
 |5
@@ Line 95: / Line 100: @@
 |0.05 g
 |0.025g
 |-
 |6
@@ Line 102: / Line 108: @@
+|
+|
 |-
 |7
@@ Line 109: / Line 116: @@
 |0.05 g
+|
 |-
 |8
@@ Line 116: / Line 124: @@
+|
 |0.025g
 |-
 |9
+|
+|
@@ Line 130: / Line 140: @@
+|
+|
 |-
 |11
@@ Line 137: / Line 148: @@
+|
+|
 |-
 |12
@@ Line 144: / Line 156: @@
 | 0.05 g
+|
 |-
 | 13
@@ Line 151: / Line 164: @@
+|
 |0.025g
 |}
 To accurately measure those values I dissolved the nutrients in water solutions and used a pipetter. However gypsum is not soluble so the quantities may be inaccurate, and Maizena clumped together, so I put clumps of it into the petri dishes.

@@ Line 4: / Line 4: @@
 === Growth environment ===
-=== Growth substrates ===
+Airflow increases homogeneity by delivering humidity and/or minerals, by eliminating micro climates or by mechanical force.
-== My own experiments ==
+Minerals in the mist increase growth speed by osmotic pressure.
-=== First test in a monotub ===
+A humid substrate means faster growth but lower density.
 First I made grain spawn out of bird seeds. Then I sterilized a box with rubbing alcohol, lined the bottom with a clean plastic film and spread sterilized substrate made of 0.75l hardwood pellets, 0.2l wheat bran and 0.75l water onto the film. I distributed some grain spawn on top of it, closed the box and waited.
 [[File:First day in the monotub.jpg|thumb|After one day, the mycelium already comes out of the grain spawn.]]
@@ Line 17: / Line 47: @@
 I let it dry and peeled it off the substrate. It was fused with the chunks of grain spawn, so I didn't get one big tissue but many small pieces, the biggest one shown in the picture. Depending on how much it was crushed it feels like paper (but more fragile) or like a very soft, light and flexible tissue. It is very thin and rips apart easily.
-==== What to improve ====
+==== What to improve====
 The grain spawn should be broken into smaller pieces using disposable gloves, and evenly mixed with the substrate. Using unpopped popcorn as grains may be a good idea since it doesn't stick together as much as bird seeds.
-=== Experimenting with substrates in petri dishes ===
+===Experimenting with substrates in petri dishes===
 I filled petri dishes with Agar, malt extract, maizena, yeast extract and gypsum according to the following table.
 {| class="wikitable mw-collapsible mw-collapsed"
@@ Line 112: / Line 142: @@
+|
+|
-|0.05 g
+| 0.05 g
+|
 |-
-|13
+| 13
 |Pellets & Bran
+|
@@ Line 124: / Line 154: @@
 To accurately measure those values I dissolved the nutrients in water solutions and used a pipetter. However gypsum is not soluble so the quantities may be inaccurate, and Maizena clumped together, so I put clumps of it into the petri dishes.
-=== Heated inoculation chamber ===
+===Heated inoculation chamber===
 It would be nice to have a chamber that can heat sterilize itself and the substrate, and then keep the temperature constant at 25 or 30° C. I am buying the XH-W1209 thermostat: https://www.berrybase.ch/xh-w1209-digitales-thermostat/temperaturschalter-12v-frei-programmierbar
-=== A very cheap CO<sub>2</sub> meter ===
+===A very cheap CO<sub>2</sub> meter===
 Since an electronic CO<sub>2</sub> sensor costs 60 CHF I make an analog one. Henry's law states that the amount of a dissolved gas in water is proportional to the partial pressure in the air above the water. Furthermore, dissolved CO<sub>2</sub> lowers the ph of water. This means that by filling a small bowl with distilled water and measuring its pH we can guess the CO<sub>2</sub> concentration of the air. Since the math are complicated, we just assume a linear (or exponential) relation and calibrate with the known atmospheric concentration and gas mixtures with CO<sub>2</sub> made from baking soda and vinegar.
 '''''pH = a + b [CO<sub>2</sub>]'''''