New SOPs - Revision history

Rachel: /* Use of the small sterile hood - not for GMOs! */

2023-11-15T14:10:42Z

Use of the small sterile hood - not for GMOs!

Rachel: /* Preventing covid-19 */

2023-11-05T16:31:56Z

Preventing covid-19

Rachel: /* Preventing covid-19 */

2023-11-05T16:31:34Z

Preventing covid-19

Rachel: /* Preventing covid-19 */

2023-11-05T16:31:02Z

Preventing covid-19

Rachel: /* Getting the best out of the current space */

2023-11-03T14:49:14Z

Getting the best out of the current space

Rachel at 14:45, 3 November 2023

2023-11-03T14:45:18Z

Rachel at 14:42, 3 November 2023

2023-11-03T14:42:14Z

Rachel at 14:41, 3 November 2023

2023-11-03T14:41:03Z

Rachel at 14:40, 3 November 2023

2023-11-03T14:40:29Z

Rachel: /* Getting the best out of the current space */

2023-11-03T14:35:40Z

Getting the best out of the current space

@@ Line 25: / Line 25: @@
 Because this hood only protects the work, not the worker, '''it must not be used for experiments with GMO organisms.'''
-Instead the gas canister in the P1 lab is used, for a sterile zone for experimental manipulation.  [https://drive.google.com/drive/folders/19hquLFedG5I9lh2Ni19sCy1Hw8qjrqFz?usp=sharing More SOPs for the lab are found here].
+'''Instead, the gas canister in the P1 lab is used, for a sterile zone for experimental manipulation.'''  [https://drive.google.com/drive/folders/19hquLFedG5I9lh2Ni19sCy1Hw8qjrqFz?usp=sharing More SOPs for the lab are found here].
 <br>

@@ Line 53: / Line 53: @@
 (final volume: 180ml)<br>
-to note, nov2023 - of course, this whole part is maybe considered too late to think about, but use of isopropyl alcohol seems a better idea than such mixes with ethanol!  (even worse is the alcohol à bruler for fondue makers?)  need to confirm if the 70% isopropyl alcohol (as we currently have in the lab) has well tested for covid too...
+to note, nov2023 - of course, this whole part is maybe considered too late to think about, but use of isopropyl alcohol seems a better idea than such mixes with ethanol!  (even worse is the alcohol à bruler for fondue makers?)  need to confirm if the 70% isopropyl alcohol (as we currently have in the lab) has been well tested for covid too...
 =Getting the best out of the current space =
 [https://wiki.hackuarium.ch/images/5/57/P1labmap.pdf Here is an overview of the P1 lab]

@@ Line 53: / Line 53: @@
 (final volume: 180ml)<br>
-to note, nov2023 - use of isopropyl alcohol seems a better idea than such mixes with ethanol!  (even worse is the alcohol à bruler for fondue makers?)  need to confirm if the 70% isopropyl alcohol (as we currently have in the lab) has well tested for covid too...
+to note, nov2023 - of course, this whole part is maybe considered too late to think about, but use of isopropyl alcohol seems a better idea than such mixes with ethanol!  (even worse is the alcohol à bruler for fondue makers?)  need to confirm if the 70% isopropyl alcohol (as we currently have in the lab) has well tested for covid too...
 =Getting the best out of the current space =
 [https://wiki.hackuarium.ch/images/5/57/P1labmap.pdf Here is an overview of the P1 lab]

@@ Line 53: / Line 53: @@
 (final volume: 180ml)<br>
+to note, nov2023 - use of isopropyl alcohol seems a better idea than such mixes with ethanol!  (even worse is the alcohol à bruler for fondue makers?)  need to confirm if the 70% isopropyl alcohol (as we currently have in the lab) has well tested for covid too...
 =Getting the best out of the current space =
 [https://wiki.hackuarium.ch/images/5/57/P1labmap.pdf Here is an overview of the P1 lab]

@@ Line 2: / Line 2: @@
 '''Standard Operating Procedures for Proper Use of Equipment in the Ecublens Space'''
-Certain Hackuarium equipment could not be fit within the laboratory spaces, in particular the microbiology hood and the ultracentrifuge (the latter especially because of needs for 370V power).  Instead, these are found around the corner from the Biolab. Please see the lab map below in the gallery for more details.
+Certain Hackuarium equipment could not be fit within the laboratory spaces, in particular the microbiology hood and the ultracentrifuge (the latter especially because of needs for 370V power).  Instead, these are found around the corner from the Biolab. Please see the detailed lab map below in the gallery for more information.<br>
 In order to use this space safely, as our Biolab is authorised to do P1 experimentation with BSL1 organisms (and selection for antibiotic resistance markers, for example), certain extra precautions should always be followed, which these Standard Operating Procedures will set out.
-A new set of simplified SOPs has been put together (2023), and laminated copies for several are available in the lab for ready inspection (in the file holder  on the left corner of an upper shelf over the long drawers between the benches, at the [https://wiki.hackuarium.ch/images/5/57/P1labmap.pdf top right in this representation] of the P1 lab.
+A new set of simplified SOPs has been put together (2023), and laminated copies for several are available in the lab for ready inspection (in the file holder on the left corner of an upper shelf over the long drawers between the benches, at the [https://wiki.hackuarium.ch/images/5/57/P1labmap.pdf top right in this representation] of the P1 lab.) If they get splashed, simply wipe them clean. =)
-<br>These include bilingual versions for:  Microbiology hood use; Glove use; Inactivation and disposal of GMOs; Sterile technique with the gas canister, and more.  They can be found [https://drive.google.com/drive/folders/19hquLFedG5I9lh2Ni19sCy1Hw8qjrqFz?usp=sharing in this folder].
+<br>These SOPs include bilingual versions for:  Microbiology hood use; Glove use; Inactivation and disposal of GMOs; Sterile technique with the gas canister, and more.  They can be found [https://drive.google.com/drive/folders/19hquLFedG5I9lh2Ni19sCy1Hw8qjrqFz?usp=sharing in this folder].
 As always, if you see any modifications that should be made to these documents, or even just in the French translation, do help us fix them!
 =Initial steps=
@@ Line 58: / Line 58: @@
 Project boxes from shelf areas are used to store project specific equipment, so things aren't left lying about the lab...  <br>Please make sure to tidy the space after manipulations, so the next people coming to do something are able to do what they need to do! <br>
-Separate out waste and follow the [https://docs.google.com/document/d/1C4M8G3qEWCTItirqRVOryD_Q1GzAvKWE02v0UlOKBE8/edit?usp=sharing safety guidelines]! Here are [https://drive.google.com/file/d/12QLUzI3QR8B3lcPZyaxbqSchJdKfDIyt/view?usp=sharing the same guidelines in French].<br>
+Separate out waste and always follow the [https://docs.google.com/document/d/1C4M8G3qEWCTItirqRVOryD_Q1GzAvKWE02v0UlOKBE8/edit?usp=sharing safety guidelines]!
 If something will be left out or on (i.e. the plates you just poured, the incubator), label with instructions, dates and initials, so no one can complain... :)
 Make sure to do risk analyses, especially in projects that involve GMOs, but also in general.  This [https://communitybio.pubpub.org/pub/m255th2q/release/1#biological-risk-assessments-for-community-laboratories community biolab document] can be of great help, when you start in on this essential risk assessment work.
-Please [https://docs.google.com/forms/d/e/1FAIpQLSfrb9lf5ezmW_mzrWHybNUIahu6KINQff0GjlG-FIt6RwE45Q/viewform use this form to help document] your risk analyses efforts and save any other analyses done. (Try this  [https://docs.google.com/spreadsheets/d/1VdjRurnfdxHt-4MwQ6NHIW-fgihJCqZnAa43uliXPFs/edit?usp=sharing possible model]  for your project.).<br>
+Please [https://docs.google.com/forms/d/e/1FAIpQLSfrb9lf5ezmW_mzrWHybNUIahu6KINQff0GjlG-FIt6RwE45Q/viewform use this form to help document] your risk analyses efforts and save any other analyses done. (Try this [https://docs.google.com/spreadsheets/d/1VdjRurnfdxHt-4MwQ6NHIW-fgihJCqZnAa43uliXPFs/edit?usp=sharing possible model]  for your project.).<br>
 === Gallery ===
-The coop space now includes 3 lab spaces for Hackuarium, plus a bit more.
+The coop space now includes 3 rooms for Hackuarium, plus a bit more.
-[https://wiki.hackuarium.ch/images/3/33/Space_Outline.pdf Here is a simplified view of the coop building...]<br>
+[https://wiki.hackuarium.ch/images/3/33/Space_Outline.pdf Here is a simplified view of the overall coop building in Ecublens...]<br>
 Maurice and his student, taking precautions in the Octanis space during the covid-19 pandemic. <br>
 Terrariums by Vanessa and Tatiana.<br> and the gorgeous orchids - that only lost their flowers finally in June 2020...

@@ Line 1: / Line 1: @@
-[[File:Hackuarium_logo15.png|100px|thumb]]
+This wiki page builds on the details in the initial [[P1 SOP]] page, which was put together initially for our old lab in Renens.[[File:Hackuarium_logo15.png|100px|thumb]]
 '''Standard Operating Procedures for Proper Use of Equipment in the Ecublens Space'''
-Certain Hackuarium equipment could not be fit within the laboratory spaces, in particular the microbiology hoods and the ultracentrifuge (the latter especially because of needs for 370V power).  Instead, these are found around the corner from the Biolab.<br>
+Certain Hackuarium equipment could not be fit within the laboratory spaces, in particular the microbiology hood and the ultracentrifuge (the latter especially because of needs for 370V power).  Instead, these are found around the corner from the Biolab. Please see the lab map below in the gallery for more details.
-A new set of simplified SOPs has been put together (2023), and laminated copies for several are available in the lab for ready inspection (in the file holder  on the left corner of an upper shelf over the long drawers between the benches, at the [https://wiki.hackuarium.ch/images/5/57/P1labmap.pdf top right in this representation] of the P1 lab.<br>These include bilingual versions for:  Microbiology hood use; Glove use; Inactivation and disposal of GMOs; Sterile technique with the gas canister, and more.  They can be found [https://drive.google.com/drive/folders/19hquLFedG5I9lh2Ni19sCy1Hw8qjrqFz?usp=sharing in this folder].
+<br>
 As always, if you see any modifications that should be made to these documents, or even just in the French translation, do help us fix them!

@@ Line 5: / Line 5: @@
 In order to use this space safely, if our Biolab is authorised to do P1 experimentation with BSL1 organisms (and selection for antibiotic resistance markers, for example), certain extra precautions should always be followed, which these Standard Operating Procedures will set out.
-A new set of simplified SOPs has been put together (2023), and laminated copies for several are available on the left corner of an upper shelf over the long drawers between the benches in the lab for ready inspection.<br>These include bilingual versions for:  Microbiology hood use; Glove use; Inactivation and disposal of GMOs; Sterile technique with the gas canister, and more.  They can be found [https://drive.google.com/drive/folders/19hquLFedG5I9lh2Ni19sCy1Hw8qjrqFz?usp=sharing in this folder].
+A new set of simplified SOPs has been put together (2023), and laminated copies for several are available in the lab for ready inspection (in the file holder  on the left corner of an upper shelf over the long drawers between the benches, at the [https://wiki.hackuarium.ch/images/5/57/P1labmap.pdf top right in this representation] of the P1 lab.<br>These include bilingual versions for:  Microbiology hood use; Glove use; Inactivation and disposal of GMOs; Sterile technique with the gas canister, and more.  They can be found [https://drive.google.com/drive/folders/19hquLFedG5I9lh2Ni19sCy1Hw8qjrqFz?usp=sharing in this folder].
 =Initial steps=
@@ Line 17: / Line 17: @@
 After all work is complete (streaking, culture inoculation etc), the materials should again be sealed in the box to be brought back to the lab space (and put in the incubator for cultivation, for example).
-Because this hood only protects the work, not the worker, it must not be used for experiments with GMO organisms.  Instead the gas canister in the P1 lab is used, for a sterile zone for experimental manipulation.  [https://drive.google.com/drive/folders/19hquLFedG5I9lh2Ni19sCy1Hw8qjrqFz?usp=sharing More SOPs for the lab are found here].
+Because this hood only protects the work, not the worker, '''it must not be used for experiments with GMO organisms.'''
 <br>