Normalfreezer expt - Revision history

Rachel: /* Third Test */

2017-11-24T17:09:16Z

Third Test

2017-11-24T17:02:52Z

Conclusions

2017-11-24T17:01:42Z

2017-11-24T17:00:08Z

Third Test

2017-11-24T16:57:25Z

Gallery

2017-11-24T16:57:02Z

Second Test

2017-11-24T12:39:38Z

Third Test

2017-11-03T15:09:00Z

2017-11-03T15:07:33Z

First Test

2017-11-03T15:07:17Z

@@ Line 26: / Line 26: @@
 =Third Test=
 Did another round of plate streaking on 22nov17 with both '1' vials - glycerol vs milk stock test.<br>
-Noticed that the vial for the milk had a 'special' layer at the top (0.4mm)...  and this was where I got the innoculum<br>
+Noticed that the vial for the milk had a 'special' layer at the top (0.4mm)...  and this was where I got the innoculum.<br>
-Perhaps this stock also grew less well this time - for sure... <br>
+Perhaps this stock also grew less well this time - but certainly not sure... <br>
 Next time, should take out innoculu frozen, with the loop, as usual, but then let the drop thaw in a sterile tube, so can put the same volumes on the two plates for the streaks. <br>

← Older revision		Revision as of 17:02, 24 November 2017
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−	[http://wiki.hackuarium.ch/w/P1_Lab_Activities#List_of_P1_Experiments =Back to P1 experiment list=]	+	[http://wiki.hackuarium.ch/w/P1_Lab_Activities#List_of_P1_Experiments *Back to P1 experiment list]

← Older revision		Revision as of 17:01, 24 November 2017
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	=Conclusions=		=Conclusions=
		+
		+
		+	[http://wiki.hackuarium.ch/w/P1_Lab_Activities#List_of_P1_Experiments =Back to P1 experiment list=]

@@ Line 26: / Line 26: @@
 =Third Test=
 Did another round of plate streaking on 22nov17 with both '1' vials - glycerol vs milk stock test.<br>
-Noticed that the vial for the milk had a 'special' layer at the top (0.4mm)... <br>
+Noticed that the vial for the milk had a 'special' layer at the top (0.4mm)...  and this was where I got the innoculum<br>

@@ Line 32: / Line 32: @@
-<gallery mode=packed widths=300 heights=300>
+<gallery mode=packed widths=400 heights=400>
 File:IMG_2084.JPG
@@ Line 38: / Line 38: @@
 File:IMG 2104.JPG
 File:IMG 2105.JPG
 </gallery>

@@ Line 22: / Line 22: @@
 =Second Test=
 On 2 November, streaked out 2 plates with the frozen cultures.<br>
-Both grew very well!<br>
+Both grew very well, as seen in the third and fourth images of the gallery below!<br>
 =Third Test=

← Older revision		Revision as of 12:39, 24 November 2017
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	=Third Test=		=Third Test=
−	~~To try~~ another plate streaking ~~in one month, about 5Dec17~~...	+	Did another round of plate streaking on 22nov17 with both '1' vials - glycerol vs milk stock test.<br>
		+	Noticed that the vial for the milk had a 'special' layer at the top (0.4mm)... <br>

← Older revision		Revision as of 15:09, 3 November 2017
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	On 2 November, streaked out 2 plates with the frozen cultures.<br>		On 2 November, streaked out 2 plates with the frozen cultures.<br>
	Both grew very well!<br>		Both grew very well!<br>
		+
		+	=Third Test=
		+	To try another plate streaking in one month, about 5Dec17...

@@ Line 9: / Line 9: @@
 <br>
 =First Test=
-We wanted to test the method of Cody et al 2008, J Microbial Methods. testing 10% skim milk for stocking bacterial strains vs. the normal 15% glycerol conditions.
+We wanted to test the method of Cody et al 2008, J Microbial Methods. testing 10% skim milk for stocking bacterial strains vs. the normal 15% glycerol conditions.<br>
 The bacterial strain DH5alpha was inoculated on 15oct and grown in LB o/n at 37<sup>o</sup>C with shaking in LB media.<br>
 To note: you should not autoclave milk powder!!
@@ Line 21: / Line 19: @@
 Made 2 big Nunc freezing tubes with each combination, 1/2 E coli culture and 1/2 milk or glycerol. <br>
 Put all 4 vials into the freezer in a styrofoam chunk to let freeze gradually. <br>
 =Second Test=

← Older revision		Revision as of 15:07, 3 November 2017
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		+	=== Gallery ===
		+
		+
		+	<gallery mode=packed widths=300 heights=300>
		+
		+	File:IMG_2084.JPG
		+	File:IMG_2085.JPG
		+	File:IMG 2104.JPG
		+	File:IMG 2105.JPG
		+
		+	</gallery>
		+	<br>

	=Conclusions=		=Conclusions=