P1 SOP - Revision history

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2024-05-02T09:12:01Z

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AurélienJ: /* SOP in the Lab - Standard Operating Procedure in English */

2024-05-02T09:07:39Z

SOP in the Lab - Standard Operating Procedure in English

Rachel: /* SOP in the Lab - Standard Operating Procedure in English */

2024-05-02T08:53:58Z

SOP in the Lab - Standard Operating Procedure in English

Rachel at 08:53, 2 May 2024

2024-05-02T08:53:03Z

Rachel at 05:43, 27 November 2023

2023-11-27T05:43:48Z

Rachel at 23:22, 20 November 2023

2023-11-20T23:22:20Z

Rachel at 14:54, 5 November 2023

2023-11-05T14:54:56Z

Rachel: /* SOP in the Lab - Standard Operating Procedure in English */

2023-11-03T13:30:02Z

SOP in the Lab - Standard Operating Procedure in English

Rachel at 13:29, 3 November 2023

2023-11-03T13:29:06Z

Rachel: /* SOP in the Lab - English */

2017-10-04T16:46:02Z

SOP in the Lab - English

← Older revision		Revision as of 09:12, 2 May 2024
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−	French version here - Version francaise ici.	+	French version [[SOP du laboratoire P1\|here]] - Version francaise ici.

	== SOP in the Lab - Standard Operating Procedure in English ==		== SOP in the Lab - Standard Operating Procedure in English ==

← Older revision		Revision as of 09:07, 2 May 2024
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		+	French version here - Version francaise ici.

	== SOP in the Lab - Standard Operating Procedure in English ==		== SOP in the Lab - Standard Operating Procedure in English ==

@@ Line 3: / Line 3: @@
 '''Introduction:'''<br>
-This document describes the basic standard operating procedure (SOP) for activities in Hackuarium’s P1 lab. It covers behavior, safety, storage and protocols that are to be followed in the lab space. Each user has to read the procedure and approve it in order to participate in any kind of activities within the space. This document was begun by Yann Pierson (thanks!!) and inspired by the World Health Organization. Laboratory biosafety manual. – 3rd ed  More recently, we have put together a folder of SOPs printed out and laminated, that can be found in [https://drive.google.com/drive/folders/1tYFvOUAO7kB6p8DdItHEARoXE0QKFYqU?usp=sharing pdf versions of main SOPs here].  Just say if more are needed, especially with different projects...<br>
+This document describes the basic standard operating procedure (SOP) for activities in Hackuarium’s P1 lab. It covers behavior, safety, storage and protocols that are to be followed in the lab space. Each user has to read the procedure and approve it in order to participate in any kind of activities within the space. This document was begun by Yann Pierson (thanks!!) and inspired by the World Health Organization. Laboratory biosafety manual.
 <br>
 '''Behavior in the P1 laboratory:'''<br>
 The lab is a small space, a maximum of six collaborators can perform manipulations at any time. Another collaborator can enter only to supervise, request or take an item of his own. Collaborators have to communicate and avoid any collisions. ''A calm and relaxed state of mind has to be observed when performing activities.'' Utmost cleanliness is essential after and during any work. Benches have to be cleaned after manipulations, with 70% ethanol and should be spotless to avoid any contamination. The material in the lab should generally stay there for lab use, and not be taken into the rest of the Hackuarium (biohacker space). It is strongly encouraged to thoroughly wash hands when experiments are over. Food and beverages are strictly forbidden. <br>
-See [https://docs.google.com/document/d/1C4M8G3qEWCTItirqRVOryD_Q1GzAvKWE02v0UlOKBE8/edit?usp=sharing this page first], which lists basic rules, if you would like to do P1 experiments at Hackuarium, and talk to the Biosafety Officer (Rachel) or the deputy (Javier)! <br>
+See [https://docs.google.com/document/d/1C4M8G3qEWCTItirqRVOryD_Q1GzAvKWE02v0UlOKBE8/edit?usp=sharing this page first], which lists basic rules, if you would like to do P1 experiments at Hackuarium, and talk to the Biosafety Officer (Rachel) or the deputy (Aurélien)! <br>
 <br>
 '''Safety:'''<br>

@@ Line 3: / Line 3: @@
 '''Introduction:'''<br>
-This document describes the standard operating procedure (SOP) for activities in Hackuarium’s P1 lab. It covers behavior, safety, storage and protocols that are to be followed in the lab space. Each user has to read the procedure and approve it in order to participate in any kind of activities within the space. This document was begun by Yann Pierson (thanks!!) and inspired by the World Health Organization. Laboratory biosafety manual. – 3rd ed <br>
+This document describes the basic standard operating procedure (SOP) for activities in Hackuarium’s P1 lab. It covers behavior, safety, storage and protocols that are to be followed in the lab space. Each user has to read the procedure and approve it in order to participate in any kind of activities within the space. This document was begun by Yann Pierson (thanks!!) and inspired by the World Health Organization. Laboratory biosafety manual. – 3rd ed  More recently, we have put together a folder of SOPs printed out and laminated, that can be found in [https://drive.google.com/drive/folders/1tYFvOUAO7kB6p8DdItHEARoXE0QKFYqU?usp=sharing pdf versions of main SOPs here].  Just say if more are needed, especially with different projects...<br>
 <br>
 '''Behavior in the P1 laboratory:'''<br>

@@ Line 74: / Line 74: @@
 <br>
 Solid wastes:<br>
-The solid wastes (non perforant material such as needles, shards or items that might damage the bag) are to be disposed after disinfection in the normal waste for incineration. <s>in the closed container containing the biowaste bag. Once the container is full, close the bag with a plastic clamp. In doubt of damaged bag, you can put it in a second biowaste bag. Autoclave the bag for 30 min at 121°C. The autoclaved bag has to be placed in a normal trash bag, closed with a plastic clamp and trashed via the usual elimination route.</s> <br>
+The solid wastes (non perforant material such as needles, shards or items that might damage the bag) are to be disposed after disinfection in the normal waste for incineration. <s>in the closed container containing the biowaste bag. Once the container is full, close the bag with a plastic clamp. In doubt of damaged bag, you can put it in a second biowaste bag. Autoclave the bag for 30 min at 121°C. The autoclaved bag has to be placed in a normal trash bag, closed with a plastic clamp and trashed via the usual elimination route.</s>  In Ecublens, no big autoclave is available for such a method.  We [https://drive.google.com/file/d/1_db3H7Ybm_kd7yV-wnMKF55Snr805TAp/view?usp=sharing inactivate GMOs, generally by bleach treatment], for safe disposal in the garbage, which is furthermore incinerated in Switzerland.<br>
 <br>
 '''Potentially dangerous instruments:'''<br>

@@ Line 106: / Line 106: @@
 Mutagenicity<br>
 History of other laboratories working with the same organism<br>
-measures should be taken to minimise these risks<br>
+Measures should be taken to minimise risks<br>
 Produce a detailed record of what you are about to do (continue doing so during the experiment).<br>
-Decide whether to notify the authorities, this can be [https://www.ecogen.admin.ch/ecogen/Forms/LogOn/LogOnPage.aspx done online on This page].<br>
+Decide whether to notify the authorities.<br>
 Notify the authorities about any changes while the experiment is being performed.<br>
 After completion of the project, update the status of the experiment.<br>

← Older revision		Revision as of 16:46, 4 October 2017
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−	== SOP in the Lab - English ==	+	== SOP in the Lab - Standard Operating Procedure in English ==

	'''Introduction:'''<br>		'''Introduction:'''<br>