Pushing the P1 - Revision history

Rachel: /* Previous situation */

2023-10-03T05:23:33Z

Previous situation

Rachel: /* A P1 Lab for Open and Accessible DIT-Research */

2023-10-02T21:44:01Z

A P1 Lab for Open and Accessible DIT-Research

Rachel: /* A P1 Lab for Open and Accessible DIT-Research */

2023-10-02T12:44:19Z

A P1 Lab for Open and Accessible DIT-Research

Rachel at 12:41, 2 October 2023

2023-10-02T12:41:55Z

Hekkcess at 15:11, 17 November 2017

2017-11-17T15:11:49Z

Rachel: /* Microbes */

2017-11-01T16:18:06Z

Microbes

Rachel: /* Abbreviations */

2017-11-01T16:17:38Z

Abbreviations

Rachel: /* A P1 Lab for Open and Accessible DIT-Research */

2017-11-01T16:17:09Z

A P1 Lab for Open and Accessible DIT-Research

Rachel at 16:16, 1 November 2017

2017-11-01T16:16:33Z

Rachel at 16:15, 1 November 2017

2017-11-01T16:15:11Z

@@ Line 14: / Line 14: @@
 Additionally, 6 people (including Dan and Thomas) have currently taken the intial P1 user intro to biosafety course, discussed further below.
-'''Big News (update, 1nov17)'''
+'''Old Renens News (update, 1nov17)'''
 Our first bacterial strains are now growing on plates in the incubator!  <br>
 Looking forward to the seeing bioluminescence from one of these strains, and fluorescence (esp red) from the other! <br>
 [https://docs.google.com/spreadsheets/d/1xPDdciRnwBRrdXu_wT6FX3BfO8Sp1qZ6EAoU31n5PQc/edit?usp=sharing Here] is the current list of strains in the lab.<br>
 [https://docs.google.com/spreadsheets/d/1EwJ2i03dDeRm01-yQTxCNMZSTpsEWj0K418y1FhTjLo/edit#gid=0 Here] the current list of P1 lab users. <br>
@@ Line 22: / Line 24: @@
 Talk to the biosafety officer, Rachel Aronoff, and deputy biosafety officer, Olivier Emery, about your plans in advance! <br>
 Documentation on the wiki is highly encouraged. <br>
 ==Abbreviations ==

@@ Line 2: / Line 2: @@
 Having a laboratory and instruments is one thing, possessing the tools to do actual biology research is another thing. For over three years, the Hackuarium community has built a space where people can work on projects related to biology among other fields. The ambition of the international DIY biology scene is to bring to the people the tools to perform and work on the whole range of biological applications, as qualitatively and cheaper than what the industrial or academic institutions do. To achieve such level, the Hackuarium lab has finally been renovated, to include a space that is upgraded to a higher level of competency, for experimentation at the P1 biosafety level where genetic manipulation opens the door to a vast range of research topic and engineering opportunities.<br>
 We plan P1 work to start out in bacteria. Depending on future demands, we can imagine adding other activities.<br>
-[[File:P1labplan version0.2 270917.jpg|400px|thumb|center|THE P1 LAB CONFIGURATION back in Renens (ca. 2018)]]<br>
+[[File:P1lab2023.png|400px|thumb|center|The current P1 Lab in the Coop Space in Ecublens.]]<br>  [[File:P1labplan version0.2 270917.jpg|400px|thumb|center|THE P1 LAB CONFIGURATION back in Renens (ca. 2018)]]<br>
 <br>
 ==Previous situation==

@@ Line 45: / Line 45: @@
 ==Primers==
 * [http://www.microsynth.ch/ Microsynth] : ~ 10.-/30 bp primer
 ==Sequencing==
 *[http://www.gatc-biotech.com/fr/lp/sequencage-nextgen.html?gclid=CKzRrtTbm80CFdiZGwodBycOaA GATC]
 ==Genes==

@@ Line 2: / Line 2: @@
 Having a laboratory and instruments is one thing, possessing the tools to do actual biology research is another thing. For over three years, the Hackuarium community has built a space where people can work on projects related to biology among other fields. The ambition of the international DIY biology scene is to bring to the people the tools to perform and work on the whole range of biological applications, as qualitatively and cheaper than what the industrial or academic institutions do. To achieve such level, the Hackuarium lab has finally been renovated, to include a space that is upgraded to a higher level of competency, for experimentation at the P1 biosafety level where genetic manipulation opens the door to a vast range of research topic and engineering opportunities.<br>
 We plan P1 work to start out in bacteria. Depending on future demands, we can imagine adding other activities.<br>
-[[File:P1labplan version0.2 270917.jpg|400px|thumb|center|THE CURRENT LAB CONFIGURATION]]<br>
+[[File:P1labplan version0.2 270917.jpg|400px|thumb|center|THE P1 LAB CONFIGURATION back in Renens (ca. 2018)]]<br>
 <br>
-==Current situation==
+==Previous situation==
-The authorities have now been notified that our P1 activities will officially begin on 1november2017.<br>
+The authorities were initially notified that our P1 activities would officially begin on 1november2017.<br>
-Acknowledgement of this info has been [https://drive.google.com/open?id=0B1jaPHOkH_42ODVtY2NNSVF0TUk received from bafu], but we still need to organise a few more things in the lab, finalise the map, and find a deputy biosecurity officer to help Rachel.<br>
+Acknowledgement of this info has been [https://drive.google.com/open?id=0B1jaPHOkH_42ODVtY2NNSVF0TUk received from bafu], but we still need to organise a few more things in the lab, finalise the map, and find a deputy biosecurity officer to help Rachel. (initially this was Olivier, and later Javier took on this role)<br>
 Additionally, getting this documentation also in French could be quite important for everyone to benefit. <br>
 (thanks in advance for some help!) <br>
@@ Line 318: / Line 318: @@
 *Good old openTaq
 *DNA fragment purification by gel extraction. (cheap, easy, no need of DPNI enzymes)
-*Ligation through modified Gibson (lysed bacterial supernatant + enzymatic complementation (Exonuclease mostly), To investigate further see the [[Media:SLiCE.PDF | SLiCE]] method)
+*Ligation through modified Gibson (lysed bacterial supernatant + enzymatic complementation (Exonuclease mostly), To investigate further see the [[Media:SLiCE.PDF| SLiCE]] method)
 *Transformation in chemo competent cells by heat shock (way cheaper than electro-competent cells)
 *Usual LB-agar plating with ampicilin selection

@@ Line 2: / Line 2: @@
 Having a laboratory and instruments is one thing, possessing the tools to do actual biology research is another thing. For over three years, the Hackuarium community has built a space where people can work on projects related to biology among other fields. The ambition of the international DIY biology scene is to bring to the people the tools to perform and work on the whole range of biological applications, as qualitatively and cheaper than what the industrial or academic institutions do. To achieve such level, the Hackuarium lab has finally been renovated, to include a space that is upgraded to a higher level of competency, for experimentation at the P1 biosafety level where genetic manipulation opens the door to a vast range of research topic and engineering opportunities.<br>
 We plan P1 work to start out in bacteria. Depending on future demands, we can imagine adding other activities.<br>
-[[File:P1labplan version0.2 270917.jpg|800px|thumb|left|THE CURRENT LAB CONFIGURATION]]<br>
+[[File:P1labplan version0.2 270917.jpg|400px|thumb|center|THE CURRENT LAB CONFIGURATION]]<br>
 <br>
 ==Current situation==

@@ Line 29: / Line 29: @@
 ==Microbes==
-We have only the right to work with organisms classified as BSL1 according to Swiss Law.
+We now have the right to work with organisms classified as BSL1 according to Swiss Law.
 The first step is to check if the microbe you want to work is BSL1, go to https://www.bafu.admin.ch/bafu/fr/home/themes/biotechnologie/publications-etudes/publications/classification-des-organismes.html

@@ Line 26: / Line 26: @@
 ==Abbreviations ==
-Biological safety level 1 lab (P1), biosafety officer (BSO), standard operating procedure (SOP)
+Biological safety level 1 lab (P1), biosafety officer (BSO), standard operating procedure (SOP)...
 ==Microbes==

@@ Line 1: / Line 1: @@
-=Introduction=
+=A P1 Lab for Open and Accessible DIT-Research =
 Having a laboratory and instruments is one thing, possessing the tools to do actual biology research is another thing. For over three years, the Hackuarium community has built a space where people can work on projects related to biology among other fields. The ambition of the international DIY biology scene is to bring to the people the tools to perform and work on the whole range of biological applications, as qualitatively and cheaper than what the industrial or academic institutions do. To achieve such level, the Hackuarium lab has finally been renovated, to include a space that is upgraded to a higher level of competency, for experimentation at the P1 biosafety level where genetic manipulation opens the door to a vast range of research topic and engineering opportunities.<br>
 We plan P1 work to start out in bacteria. Depending on future demands, we can imagine adding other activities.<br>

@@ Line 25: / Line 25: @@
-= Abbreviations =
+==Abbreviations ==
 Biological safety level 1 lab (P1), biosafety officer (BSO), standard operating procedure (SOP)
-===Microbes===
+==Microbes==
 We have only the right to work with organisms classified as BSL1 according to Swiss Law.
@@ Line 44: / Line 44: @@
 *Czech Collection of Microorganisms (CCM) http://www.sci.muni.cz/ccm/index.html
-===Primers===
+==Primers==
 * [http://www.microsynth.ch/ Microsynth] : ~ 10.-/30 bp primer
-===Sequencing===
+==Sequencing==
 *[http://www.gatc-biotech.com/fr/lp/sequencage-nextgen.html?gclid=CKzRrtTbm80CFdiZGwodBycOaA GATC]
-===Genes===
+==Genes==
-===Norms===
+==Following Norms==
 <br><br>
 ===Define the planned experiments (Daniel for HTGAA Academy, Rachel bio-luminescence)===