Test by YP - Revision history

Sam at 09:05, 10 February 2016

2016-02-10T09:05:06Z

Sam at 09:02, 10 February 2016

2016-02-10T09:02:33Z

Sam at 08:58, 10 February 2016

2016-02-10T08:58:28Z

Hekkcess at 08:42, 10 February 2016

2016-02-10T08:42:28Z

Hekkcess: Created page with "{{GMO Project |What is the purpose of the GMO / protein you want to create?=I need a bioluminescence for a art project (buffer test: aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa..."

2016-02-10T08:40:12Z

Created page with "{{GMO Project |What is the purpose of the GMO / protein you want to create?=I need a bioluminescence for a art project (buffer test: aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa..."

New page

{{GMO Project
|What is the purpose of the GMO / protein you want to create?=I need a bioluminescence for a art project (buffer test: aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa)
|Describe why you require lab access to achieve your goal?=I will encode the Lux operon in e.coli
|Which plasmid will you use?=pET-51b(+)
|Plasmid cassette code=Test of real cassette length (note we could restrict this field te the atgc chars): TGGAGCCACCCGCAGTTCGAAAAGGGTGCAGATGACGACGACAAGGTACCGCATATGGATACCGTTCCGTGGTTTCCGCGTACCATTCAAGAACTGGATCGTTTTGCAAATCAGATTCTGAGCTATGGTGCCGAACTGGATGCAGATCATCCGGGTTTTAAAGATCCGGTTTATCGTGCACGTCGTAAACAGTTTGCAGATATCGCATATAACTATCGTCATGGTCAGCCGATTCCGCGTGTTGAATATATGGAAGAAGAGAAAAAAACCTGGGGCACCGTGTTTAAAACACTGAAAAGCCTGTATAAAACCCACGCCTGCTATGAATATAACCATATTTTTCCGCTGCTGGAAAAATACTGCGGCTTTCATGAAGATAACATCCCGCAGCTGGAAGATGTTAGCCAGTTTCTGCAGACCTGTACCGGTTTTCGTCTGCGTCCGGTTGCAGGTCTGCTGAGCAGCCGTGATTTTCTGGGTGGTCTGGCATTTCGTGTTTTTCATTGTACCCAGTATATTCGCCATGGTAGCAAACCGATGTATACACCGGAACCGGATATTTGTCATGAACTGCTGGGTCATGTTCCGCTGTTTAGCGATCGTAGCTTTGCACAGTTTAGCCAAGAAATTGGTCTGGCCAGCCTGGGTGCACCGGATGAATATATTGAAAAACTGGCAACCATCTACTGGTTCACCGTTGAATTTGGTCTGTGTAAACAGGGCGATAGCATTAAAGCCTATGGTGCTGGTCTGCTGTCTAGCTTTGGTGAACTGCAGTATTGTCTGAGCGAAAAACCGAAACTGCTGCCGCTGGAACTGGAAAAAACCGCAATTCAGAATTATACCGTGACCGAATTTCAGCCGCTGTATTACGTTGCAGAAAGTTTTAATGATGCCAAAGAAAAAGTGCGCAATTTCGCAGCAACCATTCCGCGTCCGTTTAGCGTTCGTTATGATCCGTATACCCAGCGTATTGAAGTTCTGGATAATACCCAGCAACTGAAAATTCTGGCAGATAGCATCAATAGCGAAATTGGTATTCTGTGTAGCGCACTGCAGAAAATCAAAGGAGCTCCGGGCTTCTCCTCAATTTCCGCTCATCACCACCATCATCACCATCACCACCACT
|Peptide translation of above cassette=WSHPQFEKGADDDDKVPHMDTVPWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYMEEEKKTWGTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTCTGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCLSEKPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSINSEIGILCSALQKIKGAPGFSSISAHHHHHHHHHH
|If you want to use several building blocks for you proteins, give details on the templates=temp 1: ypp1: contains the pET-51b(+) vector, ypp2: contains the ...
|Give the primers you are going to use to realise your clones=P1_F : TGGTAGTGAAGCAAGCGCCAGCATTATTG, tm = 66°C
}}

@@ Line 15: / Line 15: @@
 - several entries for primers as [name] [sequence] [tm (melting temperature)]
-<pmap id="test" restriction-enzymes="ecori" >
+<pmap id="test" restriction-enzymes="ecori" gb-location="http://www.ncbi.nlm.nih.gov/nuccore/13937413?report=genbank">
 <pmap/>
 <div id="plasmidtest">test</div>

@@ Line 15: / Line 15: @@
 - several entries for primers as [name] [sequence] [tm (melting temperature)]
-<pmap>
+<pmap id="test" restriction-enzymes="ecori" >
-ACTAAGCTGCCGGGTTTGAAACACGGATGATCTCGCGGAGGGTAGCATGTTGATTGTAACGATGACAGAG
+<pmap/>
-CGTTGCTGCCTGTGATCAATTCGGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAAT
-GCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGT
+<div id="plasmidtest">test</div>

← Older revision		Revision as of 08:58, 10 February 2016
Line 14:		Line 14:
	- several entries for tamplate as [name] [description]		- several entries for tamplate as [name] [description]
	- several entries for primers as [name] [sequence] [tm (melting temperature)]		- several entries for primers as [name] [sequence] [tm (melting temperature)]
		+
		+	<pmap>
		+	ACTAAGCTGCCGGGTTTGAAACACGGATGATCTCGCGGAGGGTAGCATGTTGATTGTAACGATGACAGAG
		+	CGTTGCTGCCTGTGATCAATTCGGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAAT
		+	GCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGT
		+	GGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAA
		+	GCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCC
		+	CTAAAACAAAGTTAAACATCATGGGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGT
		+	TGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGC
		+	GGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGC
		+	GAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGA
		+	AGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGA
		+	GAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCT
		+	TGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGT
		+	TCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCT
		+	GGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGC
		+	CGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGC
		+	TAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTC
		+	CACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAATGTCTAGCTAGAAATTCGTTCAAGCCGA
		+	CGCCGCTTCGCGGCGCGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTA
		+	TCAGGTCAAGTCTGCTTTTATTATTTTTAAGCGTGCATAATAAGCCCTACACAAATTGGGAGATATATCA
		+	TGCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAG
		+	GATCTTCTGCCGGTAACATGAGCAAAGTCTGCCGCCTTACAACGGCTCTCCCGCTGACGCCGTCCCGGAC
		+	TGATGGGCTGCCTGTATCGAGTGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTTGGCTGGCTGGT
		+	GGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACGGATTACGGAAGACAATACGGCGGTGAGAC
		+	CAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAG
		+	ACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCCCTCGAGGGGCCGCATTAGGCAC
		+	CCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTT
		+	TCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGC
		+	ATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGA
		+	TATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTT
		+	GCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATA
		+	GTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCA
		+	CGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTAT
		+	TTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTGTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTG
		+	ATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGG
		+	CGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGA
		+	ATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATCTAGAGGATCCGGCTTAC
		+	TAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTA
		+	TACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCT
		+	ATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCC
		+	CGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTG
		+	AAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAA
		+	GAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGT
		+	GATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTTTACCCGGTGGTGCATATC
		+	GGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTtTCCGTTATCGGGGAAGAAG
		+	TGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAAT
		+	GTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACGGTCTCATGTCGGGCTTGTCTTCTCTGGTTTA
		+	AACTATCAGTGTTTGACAGGATATATTGGCGGGTAAACCTAAGAGAAAAGAGCGTTTATTAGAATAAtCG
		+	GATATTTAAAAGGGCGTGAAAAGGTTTATCCGTTCGTCCATTTGTATGTGCATGCCAACCACAGGGTTCC
		+	CCTCGGGATCAAAGTACTTTGATCCAACCCCTCCGCTGCTATAGTGCAGTCGGCTTCTGACGTTCAGTGC
		+	AGCCTTCTTCTGAAAACGACATGTCGCACAAGTCCTAAGTTACGCGACAGGCTGCCGCCCTGCCCTTTTC
		+	CTGGCGTTTTCTTGTCGCGTGTTTTAGTCGCATAAAGTAGAATACTTGCGACTAGAACCGGAGACATTAC
		+	GCCATGAACAAGAGCGCCGCCGCTGGCCTGCTGGGCTATGCCCGCGTCAGCACCGACGACCAGGACTTGA
		+	CCAACCAACGGGCCGAACTGCACGCGGCCGGCTGCACCAAGCTGTTTTCCGAGAAGATCACCGGCACCAG
		+	GCGCGACCGCCCGGAGCTGGCCAGGATGCTTGACCACCTACGCCCTGGCGACGTTGTGACAGTGACCAGG
		+	CTAGACCGCCTGGCCCGCAGCACCCGCGACCTACTGGACATTGCCGAGCGCATCCAGGAGGCCGGCGCGG
		+	GCCTGCGTAGCCTGGCAGAGCCGTGGGCCGACACCACCACGCCGGCCGGCCGCATGGTGTTGACCGTGTT
		+	CGCCGGCATTGCCGAGTTCGAGCGTTCCCTAATCATCGACCGCACCCGGAGCGGGCGCGAGGCCGCCAAG
		+	GCCCGAGGCGTGAAGTTTGGCCCCCGCCCTACCCTCACCCCGGCACAGATCGCGCACGCCCGCGAGCTGA
		+	TCGACCAGGAAGGCCGCACCGTGAAAGAGGCGGCTGCACTGCTTGGCGTGCATCGCTCGACCCTGTACCG
		+	CGCACTTGAGCGCAGCGAGGAAGTGACGCCCACCGAGGCCAGGCGGCGCGGTGCCTTCCGTGAGGACGCA
		+	TTGACCGAGGCCGACGCCCTGGCGGCCGCCGAGAATGAACGCCAAGAGGAACAAGCATGAAACCGCACCA
		+	GGACGGCCAGGACGAACCGTTTTTCATTACCGAAGAGATCGAGGCGGAGATGATCGCGGCCGGGTACGTG
		+	TTCGAGCCGCCCGCGCACATCTCAACCGTGCGGCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGC
		+	TGGCGGCCTGGCCGGCCAGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGATGTGTATT
		+	TGAGTAAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGATGCGATGAGTAAATAAACAAATACGCAAG
		+	GGGAACGCATGAAGGTTATCGCTGTACTTAACCAGAAAGGCGGGTCAGGCAAGACGACCATCGCAACCCA
		+	TCTAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCTGTTAGTCGATTCCGATCCCCAGGGCAGTGCC
		+	CGCGATTGGGCGGCCGTGCGGGAAGATCAACCGCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACC
		+	GCGACGTGAAGGCCATCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCGGACTTGGC
		+	TGTGTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGTGCAGCCAAGCCCTTACGACATATGGGCC
		+	ACCGCCGACCTGGTGGAGCTGGTTAAGCAGCGCATTGAGGTCACGGATGGAAGGCTACAAGCGGCCTTTG
		+	TCGTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGGTGAGGTTGCCGAGGCGCTGGCCGGGTACGAGCT
		+	GCCCATTCTTGAGTCCCGTATCACGCAGCGCGTGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTT
		+	CTTGAATCAGAACCCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAAATCAAAAC
		+	TCATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCACAAACACGCTAAGTGCCGGCCGTCCGAGC
		+	GCACGCAGCAGCAAGGCTGCAACGTTGGCCAGCCTGGCAGACACGCCAGCCATGAAGCGGGTCAACTTTC
		+	AGTTGCCGGCGGAGGATCACACCAAGCTGAAGATGTACGCGGTACGCCAAGGCAAGACCATTACCGAGCT
		+	GCTATCTGAATACATCGCGCAGCTACCAGAGTAAATGAGCAAATGAATAAATGAGTAGATGAATTTTAGC
		+	GGCTAAAGGAGGCGGCATGGAAAATCAAGAACAACCAGGCACCGACGCCGTGGAATGCCCCATGTGTGGA
		+	GGAACGGGCGGTTGGCCAGGCGTAAGCGGCTGGGTTGcCTGCCGGCCCTGCAATGGCACTGGAACCCCCA
		+	AGCCCGAGGAATCGGCGTGAgCGGTCGCAAACCATCCGGCCCGGTACAAATCGGCGCGGCGCTGGGTGAT
		+	GACCTGGTGGAGAAGTTGAAGGCCGCGCAGGCCGCCCAGCGGCAACGCATCGAGGCAGAAGCACGCCCCG
		+	GTGAATCGTGGCAAGCGGCCGCTGATCGAATCCGCAAAGAATCCCGGCAACCGCCGGCAGCCGGTGCGCC
		+	GTCGATTAGGAAGCCGCCCAAGGGCGACGAGCAACCAGATTTTTTCGTTCCGATGCTCTATGACGTGGGC
		+	ACCCGCGATAGTCGCAGCATCATGGACGTGGCCGTTTTCCGTCTGTCGAAGCGTGACCGACGAGCTGGCG
		+	AGGTGATCCGCTACGAGCTTCCAGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGT
		+	GTGGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATGAACCGATACCGGGAAGGG
		+	AAGGGAGACAAGCCCGGCCGCGTGTTCCGTCCACACGTTGCGGACGTACTCAAGTTCTGCCGGCGAGCCG
		+	ATGGCGGAAAGCAGAAAGACGACCTGGTAGAAACCTGCATTCGGTTAAACACCACGCACGTTGCCATGCA
		+	GCGTACGAAGAAGGCCAAGAACGGCCGCCTGGTGACGGTATCCGAGGGTGAAGCCTTGATTAGCCGCTAC
		+	AAGATCGTAAAGAGCGAAACCGGGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGCG
		+	AGATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTTTTTGATCGATCCCGGCAT
		+	CGGCCGTTTTCTCTACCGCCTGGCACGCCGCGCCGCAGGCAAGGCAGAAGCCAGATGGTTGTTCAAGACG
		+	ATCTACGAACGCAGTGGCAGCGCCGGAGAGTTCAAGAAGTTCTGTTTCACCGTGCGCAAGCTGATCGGGT
		+	CAAATGACCTGCCGGAGTACGATTTGAAGGAGGAGGCGGGGCAGGCTGGCCCGATCCTAGTCATGCGCTA
		+	CCGCAACCTGATCGAGGGCGAAGCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATTGCC
		+	CTAGCAGGGGAAAAAGGTCGAAAAGGACTCTTTCCTGTGGATAGCACGTACATTGGGAACCCAAAGCCGT
		+	ACATTGGGAACCGGAACCCGTACATTGGGAACCCAAAGCCGTACATTGGGAACCGGTCACACATGTAAGT
		+	GACTGATATAAAAGAGAAAAAAGGCGATTTTTCCGCCTAAAACTCTTTAAAACTTATTAAAACTCTTAAA
		+	ACCCGCCTGGCCTGTGCATAACTGTCTGGCCAGCGCACAGCCGAAGAGCTGCAAAAAGCGCCTACCCTTC
		+	GGTCGCTGCGCTCCCTACGCCCCGCCGCTTCGCGTCGGCCTATCGCGGCCGCTGGCCGCTCAAAAATGGC
		+	TGGCCTACGGCCAGGCAATCTACCAGGGCGCGGACAAGCCGCGCCGTCGCCACTCGACCGCCGGCGCCCA
		+	CATCAAGGCACCCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAG
		+	ACAGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTG
		+	GCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCG
		+	GCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAA
		+	AATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCG
		+	AGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAAC
		+	ATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
		+	TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATA
		+	AAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGA
		+	TACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
		+	CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTT
		+	ATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGT
		+	AACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
		+	ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAG
		+	CTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGC
		+	AGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCATCGGAAGAACGGCA<pmap/>

← Older revision		Revision as of 08:42, 10 February 2016
Line 8:		Line 8:
	\|Give the primers you are going to use to realise your clones=P1_F : TGGTAGTGAAGCAAGCGCCAGCATTATTG, tm = 66°C		\|Give the primers you are going to use to realise your clones=P1_F : TGGTAGTGAAGCAAGCGCCAGCATTATTG, tm = 66°C
	}}		}}
		+	Nice start:
		+	What could be needed:
		+	- big box for the cassette sequence
		+	- big box for translation
		+	- several entries for tamplate as [name] [description]
		+	- several entries for primers as [name] [sequence] [tm (melting temperature)]