Difference between revisions of "Instru Centrifuge"
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At hackuarium we have two centrifuge: A table one used to centrifuge solutions in 1.5ml aliquots and a large one to centrifuge 50ml Falcon Tubes. | At hackuarium we have two centrifuge: A table one used to centrifuge solutions in 1.5ml aliquots and a large one to centrifuge 50ml Falcon Tubes. | ||
| − | == | + | ==Table centrifuges == |
=== Presentation === | === Presentation === | ||
| − | + | <gallery mode="traditional"> | |
| + | File:Centrif1.jpg| Outside overview | ||
| + | File:Centrif2.jpg|Rotor overview | ||
| + | File:2mL-tubes_spin-column.JPG|Spin column tubes do not fit! :-( | ||
| + | |||
| + | </gallery> | ||
::A) Centrifuge lid (just have to pull to open) | ::A) Centrifuge lid (just have to pull to open) | ||
::B) Time Setup [minutes] | ::B) Time Setup [minutes] | ||
::C) Speed Setup (Rpm = revolutions Per Minute) | ::C) Speed Setup (Rpm = revolutions Per Minute) | ||
::D) Power Button | ::D) Power Button | ||
| − | |||
| − | |||
| − | |||
::E) Rotor lid (Just plug or pull to screwing) | ::E) Rotor lid (Just plug or pull to screwing) | ||
::F) Rotor | ::F) Rotor | ||
| Line 24: | Line 26: | ||
=== Incompatibility === | === Incompatibility === | ||
| − | Attention: unfortunately this centrifuge does not fit 2 mL tubes normally used for column | + | Attention: unfortunately this centrifuge does not fit 2 mL tubes normally used for spin column purifications. |
| − | + | ||
| + | == Standing Centrifuges == | ||
| + | |||
| + | === Thermo Sorvall RC32B+ : Presentation === | ||
| + | This large centrifuge allows users to centrifuge large quantities of medium. Our model is fitted with a swing rotor which is able to accomodate plates, 15ml tubes, 50ml tubes and bottles. | ||
| + | |||
| + | === Security === | ||
| + | ::<li> Do not go faster than 5000 RCF | ||
| + | ::<li> Be careful with to equilibrate everything you put inside! No more than 0.2 g inbalance. | ||
| + | ::<li> If something leaked make sure to clean it. The instrument sometimes heats up, biological leaks stink if not cleaned. | ||
| + | === Known issues === | ||
| + | ::<li> The "MAIN CAPACITOR FAILURE" can be solved by 1) Unplugging the centrifuge 2) Opening the front panel 3) Switching on the fuse again. Be extra careful to unplug it! 400V 16A does not forgive... | ||
[[Category:Instruments]] | [[Category:Instruments]] | ||
Latest revision as of 17:29, 25 November 2015
Intro
The basic purpose of a centrifuge if to use rotational force to precipitate solids in the bottom of a vial. It can be use to separate solids from a liquid or to do analytic tests on some material weights. More on centrifuges: http://en.wikipedia.org/wiki/Centrifuge
At hackuarium we have two centrifuge: A table one used to centrifuge solutions in 1.5ml aliquots and a large one to centrifuge 50ml Falcon Tubes.
Table centrifuges
Presentation
Outside overview
Rotor overview
Spin column tubes do not fit! :-(
- A) Centrifuge lid (just have to pull to open)
- B) Time Setup [minutes]
- C) Speed Setup (Rpm = revolutions Per Minute)
- D) Power Button
- E) Rotor lid (Just plug or pull to screwing)
- F) Rotor
RTFM = Read The F*cking Manual
Incompatibility
Attention: unfortunately this centrifuge does not fit 2 mL tubes normally used for spin column purifications.
Standing Centrifuges
Thermo Sorvall RC32B+ : Presentation
This large centrifuge allows users to centrifuge large quantities of medium. Our model is fitted with a swing rotor which is able to accomodate plates, 15ml tubes, 50ml tubes and bottles.
Security
- Do not go faster than 5000 RCF
- Be careful with to equilibrate everything you put inside! No more than 0.2 g inbalance.
- If something leaked make sure to clean it. The instrument sometimes heats up, biological leaks stink if not cleaned.
Known issues
- The "MAIN CAPACITOR FAILURE" can be solved by 1) Unplugging the centrifuge 2) Opening the front panel 3) Switching on the fuse again. Be extra careful to unplug it! 400V 16A does not forgive...